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Abalone HINT gene and protein and application of same for preparing anticarcinogenic drug

An anti-cancer drug, protein technology, applied in the fields of peptide/protein composition, application, anti-tumor drugs, etc., can solve the problems of limited development and utilization, lack of in-depth research, etc.

Inactive Publication Date: 2009-09-02
ZHEJIANG UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

However, due to the lack of in-depth research on its corresponding functional factors, especially the lack of research on some genes that are beneficial to the treatment of human tumors, its further development and utilization are limited.

Method used

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  • Abalone HINT gene and protein and application of same for preparing anticarcinogenic drug

Examples

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Embodiment Construction

[0010] The methods of molecular biology used in the following examples of the present invention are all known techniques.

[0011] Implementation steps of the present invention include:

[0012] 1. Using the total RNA of abalone hemolymphocytes as a template, construct an abalone cDNA library and obtain the complete gene nucleotide sequence of HINT.

[0013] 2. Using DNA recombination technology to clone the sequence fragment of the HINT gene coding frame into a suitable pMD18-T vector (purchased from Takara Company, Japan), and then digested with restriction endonucleases, and pET- 28(a) prokaryotic expression vector (purchased from Novagen, USA) was ligated to obtain the recombinant expression plasmid pET-28(a)-HINT.

[0014] 3. Transform the positive recombinant plasmid PET-28(a)-HINT into the expression host strain BL21 (purchased from Tiangen Company, Germany), and induce expression with isopropylthiogalactoside (IPTG) (purchased from Sangon Company, Canada) , SDS-polya...

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Abstract

The invention relates to the biological technical field and aims at providing abalone HINT gene and protein in an abalone and an application of the same for preparing an anticarcinogenic drug. The protein coded by the abalone HINT gene has amino acid sequences shown in SEQ ID NO:2. The gene coding the protein has nucleotide sequences shown in SEQ ID NO:1. The invention also provides the application of the protein for preparing the anticarcinogenic drug. The invention obtains the gene nucleotide complete sequence of the abalone HINT, c and expresses the soluble HINT protein product through constructing procaryon expressing carriers. The protein has the function of promoting hepatoma cell to be apoptotic, can be used as the component for inducing the cell apoptosis and can be applied to the preparation of the anticarcinogenic drug.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the abalone HINT gene and protein in the abalone body and the application thereof in the preparation of anticancer drugs. Background technique [0002] Histidine triad nucleotide binding protein (HINT) is a member of the histidine triad protein superfamily, a family with nucleotide transferase and hydrolase activities, crystal structure study The results showed that HINT was the ancestor of four other members of the histidine trimer protein superfamily. Since HINT was isolated in cattle and proved to have biological functions, many homologues of HINT have been cloned successively from rabbits, humans, and even plants. Studies have shown that the protein has high structural and functional similarities in lower animals and higher animals, and the conservation of structure and function indicates that HINT plays an important biological function. Research reports have shown that HINT prot...

Claims

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Application Information

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IPC IPC(8): C12N9/22C12N15/55A61K38/46A61P35/00
Inventor 吴信忠吴刘记
Owner ZHEJIANG UNIV
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