Paspalum vaginatum body cell 60Co r radial radiation induction method

A technology of somatic cells and puffins, applied in the field of plant culture and 60Cor ray radiation mutagenesis of puffin somatic cells, can solve problems such as difficulties in conventional breeding work, and achieve the effect of improving breeding efficiency and reducing chimeras.

Inactive Publication Date: 2011-05-04
JIANGSU ACAD OF AGRI SCI +2
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Problems solved by technology

Triploid sea paspalum due to pollen abortion, self-incompatibility and other obstacles (Ma Guohua, Zhao Nanxian. Paspalum cytology and reproductive biology research [J], Subtropical Plant Science, 2003, 32 (3): 5 ~9), which brings difficulties to conventional breeding

Method used

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  • Paspalum vaginatum body cell 60Co r radial radiation induction method
  • Paspalum vaginatum body cell 60Co r radial radiation induction method
  • Paspalum vaginatum body cell 60Co r radial radiation induction method

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Embodiment Construction

[0028] 1. Materials and Methods

[0029] 1.1 Test material

[0030] The granular embryogenic callus induced by the young panicle of sea paspalum variety "Adalay" (Paspalum vaginatum Sw.cv.Adalay) ("Adalay" grass seed was provided by Shenzhen Mission Hills Golf Course) was used as material (Liang Liufang, She Jianming, Wu Yingying, et al. Regeneration of Paspalum paspalum young panicles cultured in vitro[J]. Acta Grassland Sinica, 2008, 16(6): 590~593), 60 Cor rays are radiation sources.

[0031] 1.2 Medium

[0032] The basal medium consists of MS macroelements, MS trace elements (Murashige T, Skoog F. A revised medium from rapid growth and bioassays with tobacco tissue cultures [J]. Physiol plant, 1962, 15: 473~497) and B 5 Vitamins (Gamborg OL, Miller R A, Ojima K. Nutrient requirements of suspension cultures of soybean root cells [J]. Exp. Cell Res., 1968.50: 151-158), a commonly used medium in plant tissue culture, 30g of sucrose / L;

[0033] Additional hormones in cal...

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Abstract

The invention relates to a method of radiation induction of a paspalum vaginatum somatic cell by <60>Co gamma ray, belonging to the technical field of a plant cultivation method. The method uses granular embryonic callus of the paspalum vaginatum as a material and <60>Co gamma ray as a radiation source with dosage rate of 1Gy / min and radiation dosage of 60Gy, wherein the granular embryonic callus is subjected to enrichment culture on a callus successive transfer culture medium attached by 2.0mg / L of 2,4-dichlorphenoxyacetic acid and 0.05mg / L of 6-benzylaminopurine to be used as a radiation treatment material; the callus subjected to radiation treatment is transferred to a callus differential medium attached by 2.0mg / L of 6-benzylaminopurine with bud differentiation rate at about 76 percent; and the bud is transferred to a rooting medium attached by 0.5mg / L of naphthylacetic acid with seedling rate of the bud of 42 percent. The method for radiation induction of the paspalum vaginatum somatic cell by the <60>Co gamma ray is applicable to development of new variety of plants, and provides condition for developing plant cell radiation induction breeding on cellular level.

Description

technical field [0001] Sea paspalum somatic cells of the present invention 60 Cor ray radiation mutagenesis method, relates to a plant cell regeneration and 60 The radiation mutagenesis technology of Cor ray belongs to the technical field of plant cultivation methods. Background technique [0002] Sea paspalum (Paspalum vaginatum Sw.), as a turfgrass, is widely planted in tropical and subtropical regions of the world, and it is a rising star following warm-season grasses such as bermudagrass, zoysia and false thrift grass (Xie Xinming, Lu Xiaoliang. Excellent characteristics and utilization value of sea paspalum germplasm resources [J], Journal of South China Agricultural University, 2004, 25 (Supplement II): 64-67). Triploid sea paspalum due to pollen abortion, self-incompatibility and other obstacles (Ma Guohua, Zhao Nanxian. Paspalum cytology and reproductive biology research [J], Subtropical Plant Science, 2003, 32 (3): 5 ~9), which brings difficulties to conventional...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/01C12N13/00A01H4/00
Inventor 叶晓青佘建明梁流芳倪万潮董民强王松凤吴瑛瑛黄益洪汤日圣杨莉林
Owner JIANGSU ACAD OF AGRI SCI
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