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Micro-propagation method for white birch with purple leaf

A technology of white birch and purple leaf, applied in the field of micropropagation, can solve the problems of long reproductive cycle, poor genetic stability, and low multiplication efficiency of white birch, and achieve high-efficiency reproduction and large-scale promotion of planting, short reproductive cycle, and genetic stability Good results

Inactive Publication Date: 2009-08-12
NORTHEAST FORESTRY UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a micropropagation method for Birch purple leaves in order to solve the problems of long reproductive cycle, low multiplication efficiency, high cost, low survival rate and poor genetic stability of Birch purple leaves

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: the micropropagation method of birch purple leaf in this embodiment is realized by the following steps: one, the explant of birch purple leaf after cleaning is soaked for 60s in the ethanol solution of 75% with mass concentration on the ultra-clean workbench Soak in another ethanol solution with a mass concentration of 75% for 60 seconds, then rinse with sterile water for 3 to 5 times, then disinfect with a mercuric chloride solution with a mass concentration of 0.1% for 12 to 15 minutes, and then rinse with sterile water 3 to 5 times; 2. Cut the sterilized explants into stem segments with terminal buds or 2 to 3 axillary buds and a length of 1.5 to 3 cm, and then inoculate them in induction medium at a temperature of 22 to 3 cm. 26°C, humidity 70%-80%, light intensity 100-200μmol·cm -2 ·s -1 , 12~24h of light every day, every 20~30 days under the condition of changing the fresh induction medium, cultivate for 4~6 months, obtain micro-branches ...

specific Embodiment approach 2

[0015] Specific embodiment two: The difference between this embodiment and specific embodiment one is that in step one, wash and rinse with sterile water 4 times, then use a mass concentration of 0.1% mercuric chloride solution to sterilize for 14min, then rinse with sterile water 4 times. Other steps and parameters are the same as those in Embodiment 1.

[0016] In this embodiment, a drop of Tween-20 can be added to the mercuric chloride solution.

specific Embodiment approach 3

[0017] Embodiment 3: The difference between this embodiment and Embodiment 2 is that in step 2, the explants are cut into stem segments with terminal buds or 2 axillary buds and a length of 2 cm. Other steps and parameters are the same as in the second embodiment.

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Abstract

The present invention relates to a micropropagation method of purple-leaf birch, and relates to a micropropagation method. The invention settles the problems of long breeding cycle, long propagation efficiency, high cost and low survival rate. The method of the invention comprises the following steps: 1. sterilizing the explant; 2. cutting the explant to stem segment, and then executing induction cultivation for obtaining micro-branch; 3. cutting the micro-branch to stem segment and proliferating in the culture medium; and 4. executing invigoration culture to the proliferated micro-branch, sticking into a rooting substrate for executing rooting culture for finishing the micropropagation of purple-leaf birch. The micropropagation method of purple-leaf birch of the invention has the advantages of short propagation period, high efficiency and excellent genetic stability. The propagation efficiency is that the purple-leaf birch propagates for 30-40 times every 30-40 days. The method of the invention also has the advantages of simple operating technique, labor saving, time saving and low cost. The cost is saved by 30%-50% compared with the prior art. Furthermore the rooting rate and survival rate of seedling propagation equally can obtain 100%.

Description

technical field [0001] The invention relates to a micropropagation method. Background technique [0002] Purple-leaved birch (Betulapo pulifolia x Whitespire'Royal Frost') is a new variety that has been bred for a long time based on the characteristics of leaf color through repeated hybridization of European white birch and Asian white birch. Its English name is "BetulaRoyal Frost Penci-2". Purple-leaved birch is an excellent tree species suitable for viewing all year round. Its leaves remain wine red throughout spring and summer, and turn yellow, orange-yellow, or orange-red in autumn. After the leaves fall off in winter, their cinnamon to white flakes The exfoliated trunk is also of high ornamental value. Purple-leaved birch also has the characteristics of strong insect resistance, light-loving, moisture-resistant, wide adaptability, and easy planting. Because birch is often characterized by flower sterility, no seeds or seeds produced that cannot form small plants with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 杨玲沈海龙梁立东
Owner NORTHEAST FORESTRY UNIVERSITY
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