Digital microfluid micro-mixer and mixing method

Abstract

The invention discloses a digital microfluid micro mixer and a mixing method. The mixer comprises an objective table, a piezoelectric substrate, a glass slide and a movable bracket, wherein the objective table is provided with a cavity; the piezoelectric substrate is arranged in the cavity; the lower surface of the piezoelectric substrate is a working surface; the working surface is provided with a first reflecting grating, a second reflecting grating, an interdigital transducer and a first hydrophobic layer which is used for the mixing work of two digital microfluids; the first reflecting grating and the second reflecting grating are arranged on both ends of the working surface respectively; the interdigital transducer is close to the first reflecting grating; the first hydrophobic layer is positioned between the interdigital transducer and the second reflecting grating; the upper surface of the glass slide is provided with a second hydrophobic layer which is used for placing the two digital microfluids to be mixed; and the thickness of the second hydrophobic layer is larger than that of the first hydrophobic layer. The mixer and the mixing method have the advantages that two microfluids are mixed on the working surface of the piezoelectric substrate by attaching the two digital microfluids arranged on a storage area onto a mixing work area so as to improve the space operation flexibility of the digital microfluids.

Description

technical field [0001] The invention relates to a microfluid mixer in a laboratory on a chip, in particular to a digital microfluid micromixer and a mixing method. Background technique [0002] General biomedical analysis includes basic processes such as sample pretreatment, mixing, reaction, separation, and detection. These basic processes are integrated in a chip of a few square centimeters to replace traditional laboratory work, which is called a laboratory on a chip ( Lab-on-a-chip) or Micro Total Analytical System. The lab-on-a-chip can effectively overcome the experimental errors caused by manual operation in traditional laboratory work due to its small amount of sample consumption, simple operation, and the ability to accurately complete the entire process from sample preparation to result display in a relatively short period of time. There are more and more applications in chemical analysis, DNA sequencing, biological and chemical sensing, molecular separation, nucl...

AI Technical Summary

Problems solved by technology

This kind of digital microfluidic micromixer has a short mixing time, but due to the integrated microelectrode array, a programmable microelectrode switch control system needs to be configured to achieve high requirements for microfluidic manipulation; in addition, the upper plate and The lower plate is an inseparable whole, and digital microfluidics can only be mixed in the two-dimensional plane of the upper and lower plates, which limits its integration in the lab-on-a-chip and the spatial flexibility of digital microfluidics manipulation. sex

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