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Method for keeping phytoferritin stable

A plant ferritin, stable technology, applied in animal protein processing, food science, food preparation, etc., to prolong storage time, increase yield, and facilitate wide application

Inactive Publication Date: 2009-07-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of inhibiting the self-degradation of plant ferritin by adding phenylmethanesulfonyl fluoride (Phenylmethanesulfonyl fluoride, PMSF) has not been reported at home and abroad.

Method used

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  • Method for keeping phytoferritin stable
  • Method for keeping phytoferritin stable
  • Method for keeping phytoferritin stable

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 1.5ml of the above-mentioned soybean ferritin solution (the ferritin content is about 1.5mg), and divide it into six parts on average, and add 2.25, 1.75 and 0.2 μmol of PMSF (Sigma-Aldrich Co. Beijing) to three of them respectively, and the other The corresponding amount of ultrapure water was added to the three control samples, and the samples were placed in a refrigerator at 4°C for 60 days, and then analyzed by SDS-polyacrylamide gel electrophoresis (Sodium dodecylsulfate-polyacrylamide gel electrophoresis, SDS-PAGE). Stability of soybean ferritin solution. The result is as figure 1 As shown, soybean ferritin in the control sample was significantly degraded, while in the experimental samples, the stability of soybean ferritin remained good. The experimental results showed that PMSF could keep soybean ferritin stable.

Embodiment 2

[0033]Take 1.5ml of the above soybean ferritin solution (the ferritin content is about 1.5mg), and divide it into six parts on average, and add 2, 1.5 and 0.125 μmol of PMSF (Sigma-Aldrich Co. Beijing) to three of them respectively, and the other The corresponding amount of ultrapure water was added to the three control samples, and the samples were placed in a 37°C incubator for 20 days, and then analyzed by SDS-polyacrylamide gel electrophoresis (Sodium dodecylsulfate-polyacrylamide gel electrophoresis, SDS-PAGE) , to observe the stability of soybean ferritin solution. The result is as figure 2 shown.

Embodiment 3

[0035] Take 2.5ml of the above soybean ferritin solution (the ferritin content is about 2.5mg), and divide it into ten parts on average, and add 0.025, 0.25, 0.5, 1.25 and 2.25 μmol of PMSF (Sigma-Aldrich Co. Beijing ), and the corresponding amount of ultrapure water was added to the other five control samples, and the samples were placed in a 37°C incubator for 20 days, and then SDS-polyacrylamide gel electrophoresis (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SDS-PAGE) analysis to observe the stability of soybean ferritin solution. The result is as image 3 As shown, the soybean ferritin in the control sample was significantly degraded, while in the experimental samples, the greater the final concentration of PMSF added, the better the stability of soybean ferritin remained. The experimental results showed that PMSF could keep soybean ferritin stable.

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Abstract

The invention discloses a method for keeping phytoferritin stable, and belongs to the technical field of stabilizing functional food. Phenylmethylsulfonyl fluoride is added to a phytoferritin solution, so that the concentration of the solution is between 0.1 and 9 mmol / L to inhibit the degradation of the phytoferritin. The addition of the phenylmethylsulfonyl fluoride can keep good stability of the phytoferritin, so the function of iron supplementation activity is exerted effectively; and the method also increases the yield of protein, prolongs the storage time for the protein, and is favorable for wide application of the phytoferritin.

Description

technical field [0001] The invention belongs to the stabilization technology of functional food, in particular to a method for maintaining the stability of plant ferritin. Background technique [0002] Ferritin is usually a hollow spherical molecule composed of 24 subunits, with inner and outer diameters of about 8 and 12.5 nm, respectively. Ferritin includes 8 3-fold axis channels and 6 4-fold axis channels, which are responsible for the material exchange between ferritin and the external environment. Among them, the 3-fold axis channel is the main channel of iron and the site of Fe(II) oxidation, and iron ions Enter the nucleus through this channel. Ferritin widely exists in the cells of animals, plants and microorganisms, and mainly regulates the metabolic balance of iron. When intracellular Fe 2+ At high concentrations, ferritin catalyzes Fe 2+ Oxidation reaction by oxygen molecules, and the generated Fe 3+ Stored in its internal cavity, 1 molecule of ferritin can s...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/304A23L1/305A23J3/04A23L29/00A23L33/16A23L33/185
Inventor 赵广华付晓苹陈芳胡小松
Owner CHINA AGRI UNIV
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