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B19 virus VP1 unique region gene

A unique and viral technology, applied in antiviral agents, genetic engineering, plant genetic improvement, etc., can solve the problem that NS1 protein cannot be used as a vaccine

Inactive Publication Date: 2009-07-08
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

VP1 and VP2 proteins can be used as vaccine candidates, but NS1 protein cannot be used as vaccine candidates

Method used

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  • B19 virus VP1 unique region gene
  • B19 virus VP1 unique region gene
  • B19 virus VP1 unique region gene

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Embodiment Construction

[0033] The present invention uses molecular biology technology to clone a new gene of the unique region protein of VP1 of B19 virus, constructs the unique region of recombinant plasmid pQE30-VP1 and successfully expresses it, and the purified recombinant protein pQE30-VP1 can cause an immune response against B19 virus . The present invention adopts the feasibility of the process of "Expression and Purification of Human Parvovirus B19VP1 Unique Region Protein" disclosed in "Journal of Cellular and Molecular Immunology" by Li Zhihong, Zhang Guocheng, etc. in 2007, and uses the DNA of recent blood samples containing B19 virus as the screening Source, specifically according to the implementation of the following schemes to be realized.

[0034] 1 Construction of recombinant plasmid (pQE30-VP1 unique region)

[0035] 1.1 According to the gene sequence of the B19 virus, a primer pair for the unique region of VP1 is designed, and amplified by PCR; the primer pair is as follows:

[...

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PUM

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Abstract

The invention discloses a B19 virus VP1 unique region gene, and constructs a B19 procaryon to express pQE30-VP1 unique region of cloning plasmid, express and purify the recombinant protein; the recombinant protein can cause immune cells response in vitro, and produce the highly effective antibodies; the B19 virus recombination protein of the present invention can be used for antigen diagnosis and for preparing antibody and vaccine for prevention of B19 virus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the cloning of B19 virus gene, in particular to the cloning of a new gene segment of a B19 virus VP1 unique region, and the construction of a prokaryotic expression vector, expression and purification to obtain an immunogenic recombinant protein VP1 unique region. Background technique [0002] 1. B19 virus and its pathogenicity [0003] Human parvovirus B19 (Human Parvovirus B19, PVB19) was accidentally discovered in No. 19 specimen when Cossart YE used convective immunoelectrophoresis to screen the hepatitis B virus in the blood of healthy blood donors in 1975, hence the name. It is currently the only virus known to cause human disease in the Parvovirus genus of the family Parvoviridae, and it is also the DNA virus with the smallest volume and the simplest structure among animal viruses. In 1981, the virus was detected in children with sickle cell anemia and aplastic anemia, thus con...

Claims

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Application Information

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IPC IPC(8): C12N15/35C12N15/70C07K14/015C07K16/08A61K39/42A61P31/20
Inventor 张国成李志宏聂晓晶许东亮
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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