Method for preparing response type peanut essence
A peanut flavor and reactive technology, which is applied in the field of peanut flavor preparation by using peanut pods, to solve processing problems, reduce production costs, rich peanut flavor and nutrition
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Embodiment 1
[0028] Embodiment 1, the preparation method of reactive peanut essence
[0029] (1) Add 50 g of peanut meal powder crushed to 200 mesh into a 1000 mL beaker, add 500 ml of phosphate buffer solution with pH 9.0, add 5 g of alkaline protease powder (purchased from Novozymes, alkaline protease Alcalase 2.4L), 50 Stir with an electric mixer in a water bath at 100 rpm, and after hydrolysis for 2 hours, add 5 g of complex protease (purchased from Novozymes, Protamex) and continue hydrolysis for 2 hours, then take out the beaker and heat it in a boiling water bath at 100 °C for 10 minutes to inactivate the enzyme. Filter with double-layer gauze, and the hydrolyzate is concentrated to 100mL by vacuum rotary evaporation to obtain the peanut meal hydrolyzate;
[0030] (2) Preparation of peanut essence base material by thermal reaction
[0031] In a 1000mL three-necked flask, add 100mL of peanut meal hydrolyzate, add 1.6g of amino acid A (alanine Ala), 1.4g of amino acid B (aspartic aci...
Embodiment 2
[0039] Embodiment 2, the preparation method of reactive peanut essence
[0040](1) In a 1000mL beaker, add 60g of peanut meal powder crushed to 200 meshes, add 480ml of pH9.0 phosphate buffer solution, add 8g of alkaline protease powder (purchased from Novozymes, Alcalase 2.4L), 50 Stir with an electric mixer at 100 rpm in a water bath at 100°C. After hydrolysis for 2 hours, add 10 g of protease (purchased from Novozymes, Protamex) and continue hydrolysis for 2 hours. Take out the beaker and heat it in a boiling water bath at 100°C for 20 minutes to inactivate the enzyme. Filter with double-layer gauze, and the hydrolyzate is concentrated to 100mL by vacuum rotary evaporation to obtain the peanut meal hydrolyzate;
[0041] (2) Preparation of peanut essence base material by thermal reaction
[0042] In a 1000mL three-neck flask, add 100mL of peanut meal hydrolyzate, add 2g of amino acid A (alanine Ala), 1.8g of amino acid B (aspartic acid, asparagine Asx), and 2g of amino acid...
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Abstract
Description
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Application Information
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