Electro-chemical method and system for human blood type detection

Abstract

The invention relates to a method for checking human blood groups, in particular to a method for checking human ABO blood groups via electrochemical methods and a checking system. The system is composed of an electrode system and a current resistance checking system. The method comprises: adding an object sample on a modified electrode; according to the change of the alternative current resistance caused by the reaction between the red blood cells in the blood sample and the blood type antibodies fixed on the surface of the electrode, judging the blood groups. The method has simple operation and clear result, which can effectively avoid the subjective effect of operators.

Description

field of invention [0001] The invention relates to a method and system for detecting human blood type, in particular to an electrochemical detection method and system for detecting human ABO blood type by electrochemical means. Background of the invention [0002] Blood typing is the conventional method of dividing human blood into four different types, A, B, AB, and O. Currently, blood typing methods are based on cell aggregation of red blood cell suspensions. In this method, red blood cells are first isolated from the patient's serum. Mix an appropriate amount of erythrocytes with the test solution (containing anti-A serum, anti-B serum) or its dilution introduced into the test tube or on the glass slide, and then add reagents that facilitate the immune reaction and incubate, centrifuge and stir. Then visually observe under a microscope to determine whether the cells have aggregated. [0003] If the sample undergoes cell aggregation in the presence of anti-A reagent, bu...

AI Technical Summary

Problems solved by technology

[0006] Other blood group detection methods also have their own shortcomings, such as the time-consuming neutralization experiment and low sensitivity

Dissociation experiments and mixed agglutination reactions require high operating requirements, and each experiment needs to repeatedly explore the conditions, and its sensitivity is not high; synergistic agglutination reactions use IgG antibodies to sensitize SPA proteins, and determine blood types through the agglutination of staphylococci, but the reagents High storage requirements limit its application; although radioimmunoassay and enzyme-linked immunoassays have high sensitivity, they are not suitable for single-person testing, and cannot achieve on-site testing

[0007] The above-mentioned blood type analysis and judgment methods need to be completed manually by experienced technicians, so they are often affected by factors such as the experience of the technicians and work fatigue, resulting in wrong judgments

Additionally, traditional methods require the use of items such as slides, coverslips, and pipettes, as well as anti-human globulin antibodies

If used carelessly, it is likely to cause serious pollution to personnel or the environment during the experiment

It can be seen that the currently commonly used clinical blood typing methods have problems such as long testing time, high manual labor intensity, and the results are greatly affected by subjective bias and the possibility of contamination.

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