Glycine diagnosis/measuring reagent kit and glycine concentration determination method

A technology of glycine and kits, which is applied in the field of medical/food inspection and determination, and can solve problems affecting health, nutritional imbalance, etc.

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the human body consumes too much glycine, not only can it not be absorbed and utilized by the human body, but it will also break the body's absorption balance of amino acids and affect the absorption of other amino acids, resulting in nutritional imbalance and affecting health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The glycine diagnostic / measurement reagent in this embodiment is a single reagent, including:

[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0043] Stabilizer 500mmol / L

[0044] Reduced coenzyme 0.25mmol / L

[0045] Hydrogen cyanide synthase 10000U / L

[0046] Malate dehydrogenase 16000U / L

[0047] Phenazine methyl sulfate 0.4mmol / L

[0048] Pyruvate 16mmol / L

[0049] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0050] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of tested glycine sample to reagent is 1 / 25, The reaction direction is negative reaction (declining reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0051] After ad...

Embodiment 2

[0053] The glycine diagnostic / measurement reagent in this embodiment is a dual reagent, including:

[0054] Reagent 1

[0055] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0056] Stabilizer 50mmol / L

[0057] Reduced coenzyme 0.25mmol / L

[0058] Phenazine methyl sulfate 0.4mmol / L

[0059] Pyruvate 16mmol / L

[0060] Reagent 2

[0061] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 500mmol / L

[0063] Hydrogen cyanide synthase 10000U / L

[0064] Malate dehydrogenase 16000U / L

[0065] After all the reagents are dissolved and prepared, they are divided into bottles to form a liquid double reagent, which can be used directly.

[0066] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested glycine sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction ...

Embodiment 3

[0069] The glycine diagnosis / determination reagent in this embodiment is three reagents, including:

[0070] Reagent 1

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] Reduced coenzyme 0.25mmol / L

[0074] Phenazine methyl sulfate 0.4mmol / L

[0075] Pyruvate 16mmol / L

[0076] Reagent 2

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 500mmol / L

[0079] Malate dehydrogenase 16000U / L

[0080] Reagent 3

[0081] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0082] Stabilizer 500mmol / L

[0083] Hydrogen cyanide synthase 10000U / L

[0084] After all the reagents are dissolved and prepared, they are divided into bottles to form three liquid reagents, which can be used directly.

[0085] When determining the glycine concentration, set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wave...

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PUM

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Abstract

The invention relates to a kit for diagnosing / measuring glycine by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the glycine, and belongs to the technical field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, methyl sulfate phenazine, a pyruvic acid, hydrogen cyanide synthetase, malic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the glycine.

Description

Technical field [0001] The invention relates to a glycine diagnostic / determination kit, and at the same time, the invention also relates to a method for determining the concentration of glycine, which belongs to the technical field of medical / food inspection and determination. Background technique [0002] Glycine is an important fine chemical intermediate, which is widely used in pesticide, medicine, food, fertilizer, feed and other industries. According to the preparation process of glycine and the purity of the product, it can be divided into four specifications: food grade, pharmaceutical grade, feed grade and industrial grade. [0003] Among more than 20 kinds of amino acids, glycine is the simplest one. It is a component of protein and is a non-essential amino acid. The "Sanitary Standards for the Use of Food Additives" stipulates that the allowed range of glycine is seasonings and soy milk, and the maximum use amount is 1g / kg. The results of a special supervision and inspe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/68C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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