Preparation of Gymnodinium breve inhibitor

Abstract

The invention relates to a method for preparing a Gymnodinium breve inhibitor and belongs to the field of treatment of marine red tide. The method comprises the following preparation steps: firstly, a large-scale alga ulva pertusa material is pulverized and leached through anhydrous ethanol, usually, each kilogram of the ulva pertusa material uses 2 to 5 liters of the anhydrous ethanol to obtain an extract; secondly, cream obtained through condensation of the extract is dissolved in water; a supernatant fluid is taken out, is extracted through petroleum ether and is delaminated to obtain a water phase substance; and the water phase substance is extracted with n-butyl alcohol and is delaminated to obtain a water phase substance; and finally, the water phase substance is extracted with acetic ether; and the obtained acetic ether phase is the Gymnodinium breve inhibitor. The inhibitor with the concentration of between 8 and 10 milligram / litre has optimal inhibition ratio on Gymnodinium breve. The preparation method has a simple process, low preparation cost and no organic pollution; and the used reagent has low price and lower toxicity.

Description

technical field [0001] The invention belongs to the field of marine red tide treatment, and in particular relates to a preparation method of a brevis inhibitor. Background technique [0002] There are many methods to control marine red tides, mainly divided into physical, chemical and biological methods. Among them, the physical method is mainly a mechanical method, which filters out the excessively multiplied planktonic algae in seawater by machine or artificially. This kind of method is time-consuming, laborious, expensive and difficult to apply in a large area. The chemical method is mainly through the addition of inhibitors (commonly used copper ions Cu 2+ or herbicides), but such inhibitors will cause secondary pollution, pose a threat to the survival of other aquatic organisms, and destroy the balance of the marine ecosystem. Most of the currently reported extraction methods for allelochemicals that inhibit algae require the use of more expensive reagents, separation...

AI Technical Summary

Problems solved by technology

Among them, the physical method is mainly a mechanical method, which filters out the excessively multiplied planktonic algae in seawater by machine or artificially. This kind of method is time-consuming, laborious, expensive and difficult to apply in a large area.

The chemical method is mainly through the addition of inhibitors (commonly used copper ions Cu 2+ or herbicides), but such inhibitors will cause secondary pollution, pose a threat to the survival of other aquatic organisms, and destroy the balance of the marine ecosystem

Most of the currently reported extraction methods for allelochemicals that inhibit algae require the use of more expensive reagents, separation columns, etc., and their cost is high