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Double-enzyme extraction method of tea protein

An extraction method and protease technology, applied in protein food processing, plant protein processing, protein food ingredients, etc., can solve problems such as toxicity, change the nutritional properties of tea protein, and nutrient loss, achieve simple process flow and improve technical level. , the effect of easy control of the production process

Inactive Publication Date: 2009-06-24
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Alkaline extraction of tea protein has been reported in the literature, but the alkaline extraction of tea protein has its disadvantages. This method will change the nutritional properties of tea protein, and the cysteine ​​and serine of the protein will be combined to form lys-alanine; -Alanine is not only toxic, but also causes loss of nutrients

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, a kind of double-enzyme extraction method of tea protein, carries out following steps successively:

[0040] 1), material selection: take dry 1kg tea dregs (moisture content ≤ 9%) as raw material; the protein content in the tea dregs is 23.5%;

[0041] 2), add 30L of clear water to the above 1kg of tea dregs, adjust the pH value to 7.5 with 1.5mol / L NaOH solution; obtain a tea mixture;

[0042] 3) Enzyme extraction: add 20g of alkaline protease to the above tea mixture, keep stirring at 60°C for 3h; then add 10g of compound protease, keep stirring at 60°C for 3h to obtain the extract;

[0043] 4) Inactivate enzyme: heat the extract to 90°C and keep it warm for 1 minute to inactivate the enzyme;

[0044] 5), centrifugation: centrifuge the passivated extract at 4000rpm for 25min, and collect the supernatant;

[0045] 6) The supernatant is dried by a spray dryer. The spray drying conditions are: the inlet air temperature is 200°C, the outlet air temperature...

Embodiment 2

[0046] Embodiment 2, a kind of double-enzyme extraction method of tea protein, carries out following steps successively:

[0047] 1), material selection: take dry 1kg tea dregs (moisture content ≤ 9%) as raw material; the protein content in the tea dregs is 23.5%;

[0048] 2), add 35L of clear water to the above 1kg of tea dregs, adjust the pH value to 8.0 with 1mol / L NaOH solution; obtain a tea-water mixture;

[0049] 3) Enzyme extraction: first add 10g of compound protease to the above tea mixture, keep stirring at 60°C for 4h; then add 30g of alkaline protease, keep stirring at 60°C for 4h;

[0050] 4) Inactivate enzyme: heat the extract to 90°C and keep it warm for 2 minutes to inactivate the enzyme;

[0051] 5), centrifugation: centrifuge the passivated extract at 5000rpm for 20min, and collect the supernatant;

[0052]6), the supernatant was dried by a spray drier under the same drying conditions as in Example 1 to obtain 184.1 g of light brown powdery tea protein, the...

Embodiment 3

[0053] Embodiment 3, a kind of double-enzyme extraction method of tea protein, carries out following steps successively:

[0054] 1), material selection: 1kg dry tea powder (moisture content≤9%) is used as raw material; the dry tea powder is locally produced in Hangzhou, and its protein content is 21.7%;

[0055] 2), add 40L of clear water to the above-mentioned 1kg dry tea powder, adjust the pH value to 7.5 with 1mol / L NaOH solution; obtain the tea-water mixture;

[0056] 3) Enzyme extraction: firstly add 7g of compound protease to the above tea mixture, keep stirring at 55°C for 4h; then add 28g of alkaline protease, keep stirring at 55°C for 4h to obtain the extract;

[0057] 4) Enzyme inactivation: heat the extract to 100°C and keep it warm for 1 minute to inactivate the enzyme;

[0058] 5), centrifugation: centrifuge the passivated extract at 5000rpm for 25min, and collect the supernatant;

[0059] 6), the supernatant was dried by a spray drier, and the drying condition...

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Abstract

The invention discloses a double enzyme extracting method for tea protein, which comprises the following steps in order: 1) using tea as raw material; 2) adding water into the raw material and adjusting the pH value to obtain a tea water mixture; 3) adding composite protease and alkaline protease into the tea water mixture and extracting; 4) heating the extract for deactivating the enzyme; 5) centrifugally separating the deactivated extract; 6) drying the supernatant to obtain tea protein. The double enzyme extracting method of the present invention not only can obtain tea protein with high protein content, but also achieves the advantages of simple process, health and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of tea processing, in particular to a method for extracting tea protein. Background technique [0002] my country is a big tea-producing country with abundant tea resources, but there is no further processing for a large amount of tea powder, pruned leaves, and even unmarketable coarse old tea produced in the tea production process, and they are all treated as waste. At the same time, with the production of a batch of instant tea factories, tea beverage factories, and tea polyphenol factories, a large amount of tea waste residues have also been produced. Many of these by-products have not been reused, which not only causes a waste of resources, but also seriously pollutes the environment. ecosystem. Therefore, in-depth deep processing of tea can create huge social and economic benefits. [0003] The utilization of tea waste residue is generally used as feed for poultry at present. Patent 200510049878.5 di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/14A23J3/34A23L1/305
Inventor 沈莲清王忠英黄光荣吴元锋活泼
Owner ZHEJIANG GONGSHANG UNIVERSITY
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