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Technique for extracting fish protein ferritin peptide from low value sea water fish and leftover protein

A technology of seawater fish and leftovers, applied in the biological field, can solve the problems of incorrect intake of iron, poor absorption of bioavailability, etc., to prevent iron deficiency anemia, improve bioavailability, and prevent iron deficiency The effect of anemia

Inactive Publication Date: 2009-06-24
ZHEJIANG OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Iron is an essential trace element for the human body. Iron deficiency anemia often occurs due to diet and the human body itself. Although there are many iron supplement products, such as ferrous sulfate, ferrous chloride, ferrous lactate, etc. as iron nutrition Fortifiers are used in food or supplements with unsatisfactory results, which may be due to incorrect or poorly absorbed iron intake or poor bioavailability; iron bioavailability depends on the chemical properties of the element itself Form and content in food, further research has found that ferrous glycinate, ferrous methionine, ferric glycinate and other amino acid iron chelates have good bioavailability, and their unique chemical form can be used in liquid and high-fat food. Carriers are widely used in the feed industry and as food nutritional fortifiers; however, since the discovery of the theory that peptides can be absorbed by the human body as a whole, various bioactive peptides are being discovered continuously, so using the most advanced biotechnology at present, looking for and It is found that organic iron with high bioavailability, effectively solving the difficulty of iron absorption and utilization and the danger of excessive iron in the body, and having slow release characteristics, especially fish protein iron peptide is an effective way to solve human anemia, which is also the significance of the present invention where the

Method used

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  • Technique for extracting fish protein ferritin peptide from low value sea water fish and leftover protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Leftovers such as heads, skins, bones, viscera, etc. after processing fresh low-value fish or aquatic products;

[0028] (2) Pretreatment: After the raw materials are cleaned, they are pulverized with a pulverizer, and the weight ratio of 1 to 2 is added, that is, 1 part of raw material is added to 2 parts of raw material weight of water to grind into a meat slurry;

[0029] (3) Compound enzyme hydrolysis: adjust the pH of the meat slurry to be 5, add the melonase (Guangxi Nanning Pangbo Biological Products Co., Ltd., solid powder, 400,000 IU) and flavor enzyme (Guangxi Nanning Pangbo Biological Products Co., Ltd. Biological Products Co., Ltd., liquid, 200IU), the amount of enzyme was controlled to 600IU, and the temperature was 40°C for limited hydrolysis for 2 hours. Heat the hydrolyzate to 90°C and keep it for 15 minutes to kill the enzyme activity;

[0030] (4) Plate and frame filtration: adopt 300 mesh plate and frame filter to filter the enzymatic solution to...

Embodiment 2

[0034] (1) Leftovers such as heads, skins, bones, viscera, etc. after processing fresh low-value fish or aquatic products;

[0035] (2) Pretreatment: After the raw materials are cleaned, they are crushed with a pulverizer, and the weight ratio of 1 to 3, that is, 1 part of raw material is added to 3 parts of raw material weight of water, and ground into a meat slurry;

[0036] (3) Compound enzyme hydrolysis: adjust the pH of the meat slurry to be 6, and add this melonase (Guangxi Nanning Pangbo Biological Products Co., Ltd., solid powder, 400,000 IU) and flavor enzyme (Guangxi Nanning Pangbo Biological Products Co., Ltd. Biological Products Co., Ltd., liquid, 200IU), the amount of enzyme was controlled to 1200IU, and the temperature was 50°C for limited hydrolysis for 3 hours. Heat the hydrolyzate to 95°C and keep it for 15 minutes to kill the enzyme activity;

[0037] (4) Plate and frame filtration: adopt 300 mesh plate and frame filter to filter the enzymatic solution to ob...

Embodiment 3

[0041] (1) Leftovers such as heads, skins, bones, viscera, etc. after processing fresh low-value fish or aquatic products;

[0042] (2) Pretreatment: After the raw materials are cleaned, they are pulverized with a pulverizer, and the weight ratio of 1 to 5, that is, 1 part of raw material is added to 5 parts of raw material weight of water, and ground into a meat slurry;

[0043] (3) Compound enzyme hydrolysis: adjust the pH of the meat slurry to be 7, add the melonase (Guangxi Nanning Pangbo Biological Products Co., Ltd., solid powder, 400,000 IU) and flavor enzyme (Guangxi Nanning Pangbo Biological Products Co., Ltd. Biological Products Co., Ltd., liquid, 200IU), the amount of enzyme was controlled to 2400IU, and the temperature was 60°C for limited hydrolysis for 4 hours. Heat the hydrolyzate to 100°C and keep it for 15 minutes to kill the enzyme activity;

[0044] (4) Plate and frame filtration: adopt 300 mesh plate and frame filter to filter the enzymatic solution to obt...

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PUM

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Abstract

The invention discloses a technology for extracting fish protein iron peptide from the a low value seawater fish and its processing tailings. The technology comprises the following steps: grinding the material and adding 2-5 times of water; milling into slurry and regulating the pH value of the slurry to 5-7; adding papain and flavor enzyme according to the vitality ratio 1:1 controlling the enzyme amount at 600-2400IU for hydrolyzing; filtering the enzymatic hydrolyzed liquid to obtain the hydrolysate and regulating its pH value to 6-8; adding 1-5% ferrous chloride solution according to the weight / volume ratio of ultra-filtered hydrolysate : ferrous chloride solution as 50:1 for chelating while stirring continuously; keeping for 15-60 minutes for killing the activity of enzyme; spray drying and obtaining the fish protein iron peptide. The invention can be used as organic iron intensifying agent for food or health product.

Description

technical field [0001] The invention relates to a technology for extracting fish protein iron peptide from a low-value sea fish and its processed head, skin, bone, viscera and other leftover protein, belonging to the field of biotechnology. Background technique [0002] Iron is an essential trace element for the human body. Iron deficiency anemia often occurs due to diet and the human body itself. Although there are many iron supplement products, such as ferrous sulfate, ferrous chloride, ferrous lactate, etc. as iron nutrition Fortifiers are used in food or supplements with unsatisfactory results, which may be due to incorrect or poorly absorbed iron intake or poor bioavailability; iron bioavailability depends on the chemical properties of the element itself Form and content in food, further research has found that ferrous glycinate, ferrous methionine, ferric glycinate and other amino acid iron chelates have good bioavailability, and their unique chemical form can be used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04A23L1/28A23L1/304A23L1/305
Inventor 邓尚贵裘耀东杨立业夏志国谢超
Owner ZHEJIANG OCEAN UNIV
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