Method for measuring content of furostanol saponin in tribulus fruit extract

A kind of furostanol saponin, determination method technology, applied in the field of determination of furostanol saponin content in Tribulus terrestris extract, can solve the problem that the furostanol saponin content cannot be reflected intuitively and the like

Active Publication Date: 2009-06-17
陕西步长高新制药有限公司
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Problems solved by technology

[0003] The present invention will provide a method for determining furostanol saponin content in Tribulus terrestris extract, so as to overcome the problem that the measurement values ​​existing in the prior art cannot directly reflect the furostanol saponin content in Tribulus terrestris extract

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  • Method for measuring content of furostanol saponin in tribulus fruit extract
  • Method for measuring content of furostanol saponin in tribulus fruit extract
  • Method for measuring content of furostanol saponin in tribulus fruit extract

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Embodiment Construction

[0008] The present invention will be described in detail below through specific examples.

[0009] A kind of assay method of furostanol saponin content in Tribulus terrestris extract:

[0010] Take about 30 mg of the sample to be tested, weigh it accurately, put it in a 25 ml measuring bottle, add 20 ml of methanol for ultrasonic treatment (power 250W, frequency 50kHz) for 20 minutes, let it cool, add methanol to the mark, shake well, and use it as the test solution. Take another 20 mg of Tribulus terrestris saponin compound reference substance dried under reduced pressure at 60°C to constant weight, weigh it accurately, put it in a 25ml measuring bottle, add methanol to dissolve and dilute to the mark, shake well, and use it as the reference solution. Accurately draw 2ml each of the test solution and the reference solution, put them in a 10ml measuring bottle, precisely add 5ml of the improved p-dimethylaminobenzaldehyde solution, add methanol to the mark, seal it tightly, sh...

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Abstract

The invention relates to the technical field of compound assay methods, in particular to a method for determining content of furostanol saponin in a tribulus terrestris extract. The invention aims at providing the method for determining the content of the furostanol saponin in the tribulus terrestris extract to overcome the problem that determined values can not visually reflect the furostanol saponin content of the tribulus terrestris extract in the prior art. In order to overcome the problem of the prior art, the technical solution of the method is as follows: the method for determining the content of the furostanol saponin in the tribulus terrestris extract comprises the following steps: taking samples to be determined, adding methanol for ultrasonic processing, adding the methanol after cooling to obtain a test solution; taking a furostanol saponin compound of the tribulus terrestris fruit with constant weight as a reference substance, adding the methanol to obtain a reference solution; determining absorbance at a wavelength of 516nm according to the spectrophotometry (Chinese Pharmacopoeia 2000, Vol. 1, Appendix VB), and computing to obtain the furostanol saponin content.

Description

Technical field: [0001] The invention relates to the technical field of compound content determination methods, in particular to a method for determining furostanol saponin content in Tribulus terrestris extracts. Background technique: [0002] According to research data and literature data, total saponins of Tribulus terrestris have the effect of treating cardiovascular and cerebrovascular diseases, and are the main active ingredients of Tribulus terrestris. Due to the low content of furostanoid saponins in the total saponins of Tribulus terrestris, high water solubility, and not easy to crystallize, it has been proved by pharmacological tests and drug efficacy experiments that it has obvious preventive and therapeutic effects on cardiovascular and cerebrovascular diseases, so the accurate content of steroidal saponins in Tribulus terrestris Control is an important job. At present, the existing determination method is to use CoCl 2 As a reference, compare the depth of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
Inventor 刘建申
Owner 陕西步长高新制药有限公司
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