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Method for separating triptolide from thunder god vine leaves

A technology of triptolide and vine leaves, which is applied in the field of separation of triptolide, can solve the problem of low yield of triptolide, and achieve the effect of reducing the amount of sample

Inactive Publication Date: 2009-06-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Method used

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Examples

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Effect test

Embodiment 1

[0027] Grind 200g of dried Tripterygium wilfordii leaves into powder, first extract with ethanol stirring and refluxing three times, each extraction for 1 hour, each time the amount of ethanol is 800 ml, combine the ethanol extract, concentrate and evaporate to dryness to obtain the ethanol extract. The ethanol extract was dissolved with 250 ml of ethyl acetate saturated with water and 250 ml of water saturated with ethyl acetate, filtered, and left to separate layers for 24 hours, and the ethyl acetate phase was concentrated and evaporated to dryness to obtain the ethyl acetate extract. The ethyl acetate extract was dissolved with 250 ml of petroleum ether saturated with methanol and 250 ml of methanol saturated with petroleum ether and allowed to stand for 10 hours to separate the layers, and the methanol phase was evaporated to dryness to obtain the methanol extract. The methanol extract was subjected to silica gel column chromatography, and eluted with n-hexane and ethyl ac...

Embodiment 2

[0029] 200g of dried Tripterygium wilfordii leaves are ground into powder, first extracted three times with ethanol stirring and reflux, each extraction is 2 hours, and the ethanol consumption is 1000 ml each time, the ethanol extract is combined, concentrated and evaporated to dryness to obtain the ethanol extract, and the ethanol extract is used 500 ml of ethyl acetate saturated with water and 500 ml of water saturated with ethyl acetate were dissolved, filtered, and left to separate layers for 12 hours. The ethyl acetate phase was concentrated and evaporated to dryness to obtain ethyl acetate extract. The ethyl acetate extract was dissolved with 300 ml of petroleum ether saturated with methanol and 300 ml of methanol saturated with petroleum ether and allowed to stand for 2 hours to separate the layers, and the methanol phase was evaporated to dryness to obtain the methanol extract. The methanol extract was subjected to silica gel column chromatography and eluted with n-hexa...

Embodiment 3

[0031] 200g of dried Tripterygium wilfordii leaves are ground into powder, first extracted three times with ethanol stirring and reflux, each extraction is 3 hours, each ethanol consumption is 1200 milliliters, the ethanol extract is combined, concentrated and evaporated to dryness to obtain ethanol extract, and the ethanol extract is used 150 ml of ethyl acetate saturated with water and 150 ml of water saturated with ethyl acetate were dissolved, filtered, and left to separate layers for 18 hours. The ethyl acetate phase was concentrated and evaporated to dryness to obtain ethyl acetate extract. The ethyl acetate extract was dissolved with 150 ml of petroleum ether saturated with methanol and 150 ml of methanol saturated with petroleum ether and allowed to stand for 8 hours to separate the layers, and the methanol phase was evaporated to dryness to obtain the methanol extract. The methanol extract was subjected to silica gel column chromatography, and eluted with n-hexane and ...

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Abstract

The invention discloses a method for separating triptolide from thunder god vine leaves. Thunder god vine leave powders are extracted by ethanol, the ethanol is firstly distributed by an ethyl acetate-water system, the top phase is steamed to obtain ethyl acetate extractive matter, the extractive matter is then distributed by a sherwood oil- methanol system, and the bottom phase is steamed to obtain methanol extractive matter. The sampling amount of column chromatography step is reduced by the twice distribution. The eluent is steamed by simple column chromatography to obtain the crude product of triptolide, the crude product of triptolide is distributed by a quaternionic biphase system, impurities such as coloring matter, and the like are removed, the sampling amount of high-speed reverse-current coloring matter step is reduced, and the competitive product of triptolide is obtained by refining high-speed reverse-current coloring matter. The purity of the competitive product of triptolide is about 95%, and the yield is 0.008% higher than leaves. The solvent in the method does not contain chloro-solvent which is harmful to environment and human bodies.

Description

technical field [0001] The invention relates to the extraction technology of plant effective components, in particular to a method for separating triptolide from triptolide leaves. Background technique [0002] Tripterygium Wilfordii Hook.f (TWHF for short) is a woody vine of the genus Tripterygium in the family Celastraceae. It is a kind of plant of the genus Tripterygium (Tripterygium). . As a traditional Chinese medicine (TCM), Tripterygium wilfordii has been used in China for hundreds of years. It was first recorded in "Shen Nong's Materia Medica", named Mangcao, and it was recorded in detail in "Compendium Supplements". Mountain arsenic and southern snake vine, because of their high toxicity, are also used in folks to kill insects and poisonous rats. As a drug, the main pharmacological effects of Tripterygium wilfordii are immune system effects, anti-inflammatory, and anti-fertility effects. Tripterygium wilfordii extract has shown good effects in treating rheumatoid...

Claims

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Application Information

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IPC IPC(8): C07J73/00C07J75/00
Inventor 沈剑何潮洪刘明徐亚兰
Owner ZHEJIANG UNIV
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