Plastic embedding flaking method

A technology of plastic bags and embedding boxes, which is applied in the field of embedding of biological specimens, can solve the problems of saving precious treatment time, restricting the speed of film making, and consuming patients' time and money, so as to save precious treatment time and reduce costs. Time, effect of reducing financial burden

Inactive Publication Date: 2009-05-13
THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional routine operation generally takes 4 working days (note: the overnight time in >12 hours of operation is not counted in working days), which seriously restricts the production speed and has long been a difficult problem in the field of histology. difficulty
For patients who urgently need to obtain pathological results, 4 days is too long, especially for outpatients, patients seeking medical treatment from afar, or patients from other places or even abroad. The 4 days of waiting, accommodation and consumption will consume a lot of time and money for patients. money, thereby imposing a severe financial burden on patients and consuming precious treatment time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: This plastic embedding method adopts the following process steps under the high-frequency resonance of 800,000 times per second of ultrasonic waves,

[0028] 1. Fixation: Fix the tissue specimens with mercury chloride, potassium dichromate and formaldehyde mixed fixative solution for 4 hours;

[0029] 2. Decalcification: use 2% nitric acid by volume fraction to decalcify tissue samples for 20mim;

[0030] 3. Dehydration: use gradient dehydration, that is, use 60%, 70%, 80%, and 95% ethanol solutions to dehydrate the decalcified tissue samples for 10mim respectively, then dehydrate with 100% ethanol for 5min, and replace 100% ethanol After that, dehydrate for another 5min; finally, use pure acetone to absorb 4mM of residual ethanol in the tissue specimen;

[0031] 4. Soaking: Soak the dehydrated tissue specimens with hydroxyethyl methacrylate for 1.5 hours, replace with hydroxyethyl methacrylate and then soak for 1.5 hours.

[0032] 5. Embedding: (1) Take...

Embodiment 2

[0038] Embodiment 2: This plastic embedding method adopts the following process steps under the high-frequency resonance of 800,000 times per second of ultrasonic waves,

[0039] (1), fixation: fix the tissue specimen with mercuric chloride, potassium dichromate and formaldehyde mixed fixative solution for 3h;

[0040] (2), decalcification: tissue specimen decalcification 25min with volume fraction 2% nitric acid;

[0041] (3), dehydration: dehydration, use volume fraction 60%, 70%, 80%, 95%, 100% ethanol solution to decalcify the tissue sample gradient dehydration, each concentration ethanol solution dehydrates 7mim respectively, then absorbs with pure acetone 3mim residual ethanol in the tissue specimen;

[0042] (4), soaking: soak the dehydrated tissue specimen with hydroxyethyl methacrylate for 5 hours;

[0043] (5), embedding: put the tissue specimens taken out from the soaking solution into the embedding box, then add the embedding solution into the embedding box and s...

Embodiment 3

[0046] Embodiment 3: This plastic embedding method adopts the following process steps under the high-frequency resonance of 800,000 times per second of ultrasonic waves,

[0047] (1), fixation: fix the tissue specimen with mercuric chloride, potassium dichromate and formaldehyde mixed fixative solution for 5h;

[0048] (2), decalcification: tissue sample decalcification 15mim with volume fraction 2% nitric acid;

[0049] (3), dehydration: dehydration, use volume fraction 60%, 70%, 80%, 95%, 100% ethanol solution to decalcify the tissue sample gradient dehydration, each concentration ethanol solution is dehydrated 12mim respectively, then absorbs with pure acetone Residual ethanol 6mim in the tissue specimen;

[0050] (4), soaking: soak the dehydrated tissue specimen with hydroxyethyl methacrylate for 2 hours;

[0051] (5) Embedding: Put the tissue samples taken out from the soaking solution into the embedding box, then add the embedding solution into the embedding box and se...

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PUM

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Abstract

The invention discloses a myeloid tissue plastic embedding and pelletizing method which comprises the processes of fixing, deliming, dehydrating, soaking, embedding, slicing and dyeing, wherein, the processes of fixing, deliming, dehydrating and soaking are carried out under ultrasonic high frequency resonance. Compared with the traditional plastic embedding and pelletizing method, the plastic embedding and pelletizing method shortens the time from 50 hours to 15 hours approximately, saves about 3 working days and reduces the time for plastic embedding and pelletizing greatly, thereby reducing the economic burden of the patient and gaining precious time for remedying.

Description

technical field [0001] The invention relates to a method for embedding a biological sample, in particular to a method for embedding a plastic sheet. Background technique [0002] Plastic embedding means that its embedding agent is a mixed reagent of hydroxyethyl methacrylate, polyethylene glycol-400, and N-N=methylaniline, which is used to replace paraffin. Compared with paraffin sections, sections made of plastic embedding agents have the advantages of less cell shrinkage, clear structure, and high resolution. The traditional plastic-embedded sheet-making method adopts the following process steps: [0003] 1. Fixation: fix tissue specimens with mercuric chloride, potassium dichromate and formaldehyde fixative (PCF). Fixed time>12h. [0004] 2. Decalcification: decalcify the tissue specimen with 2% nitric acid by volume fraction for 2.5 hours. [0005] 3. Dehydration: Gradient dehydration, tissue specimen dehydration, dehydration with volume fraction 80% ethanol 40mim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36G01N1/30
Inventor 姚丽杨琳杨敬慈温树鹏罗建民张学军王福旭尚银涛彭华
Owner THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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