Goat pox vaccine strain expression vector
An expression vector, goat pox virus technology, applied in biochemical equipment and methods, applications, botany equipment and methods, etc., can solve problems such as spending more time and high cost
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[0031] Describe the example of the present invention below by the construction of expression vector and application method
[0032] (1) Basic method of DNA recombination
[0033] 1. Preparation and Transformation of E. coli Peptide Sensitive Cells
[0034] The preparation of Escherichia coli competent cells is prepared by calcium chloride method. The specific method refers to Sambrook J, Russell D W. Translated by Huang Peitang, Wang Jiaxi, Zhu Houchu, etc., >. 3rd Edition Translated, Beijing: Science Press, 2002: 96-98. Use 0.1mol / L mgcl2 and 0.1mol / L CaCl2. Wash by mixing 4:1. For the transformation method of competent cells, refer to the above-mentioned references.
[0035] 2. Mini-preparation of plasmids (unless otherwise specified, the configuration of reagents refers to "Molecular Cloning Experiment Guide". 3rd edition translation, Beijing: Science Press, 2002)
[0036] Refer to "Molecular Cloning Experiment Guide" translated by Huang Peitang et al. 3rd edition tran...
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