Bacterium culture medium of northern cordyceps wine

A culture medium, the technology of Cordyceps militaris, applied in fungi and other directions, can solve the problems of degradation, no fruiting bodies, and impact on yield.

Inactive Publication Date: 2009-04-29
崔学恒
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

But when Cordyceps militaris is planted with tissue separation method for 2-3 generations, the strain will degenerate, which will seriously affect the yield, and even no fruiting bodies will grow.

Method used

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Examples

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Embodiment Construction

[0009] 100g of wheat grains are crushed into coarse grains with a particle size of 0.1-0.2mm, and 200g of fresh silkworm chrysalis are ground into a paste. 2g of peptone, 5g of glucose powder, 1g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, VB 1 Mix 10mg and 150ml of water to form nutrient water, put wheat grains and fresh silkworm chrysalis into a 250ml conical flask, add nutrient water, seal the conical flask with a cotton plug, and take it out after sterilizing at 120°C for 1 hour After cooling, it is the culture medium of the Cordyceps militaris strain of the present invention.

[0010] Add 1 to 3 pieces of degraded Cordyceps militaris strains (as large as soybean grains, and the difference in particle size does not affect them) into the above-mentioned medium under aseptic conditions for inoculation and culture, and cultivate them at 20-22°C for about 15 days. After the mycelia cover the bottom of the bottle, it can be refrigerated and stored at -2 to 2°...

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Abstract

The invention relates to a culture medium for Cordyceps militaris strains, which is characterized by consisting of 100 grams of wheat grains, 200 grams of fresh silkworm chrysalis, 2 grams of peptone, 5 grams of glucose powder, 1 gram of potassium dihydrogen phosphate, 0.5 gram of magnesium sulphate, 100 milligrams of VB1 and 150 milliliters of water. In a conical flask of 250 milliliters, the wheat grains and the fresh silkworm chrysalis are added into nutritive water consisting of the peptone, the glucose powder, the potassium dihydrogen phosphate, the magnesium sulphate, the VB1 and the water; the conical flask is sealed by a cotton plug; and the mixture is sterilized for 1 hour at a temperature of 120 DEG C, and then cooled to obatin the culture medium. The culture medium has the advantages of simple and easily obtained raw materials, and simple preparation and use. Through repeated tests for dozens of times, the strains do not degenerate after the Cordyceps militaris strains are cultured by the culture medium. By contrast, after degenerate strains are inoculated to the culture medium to generate strains, the strains are prepared into liquid strains to plant, so the culture medium can improve the yield effectively.

Description

technical field [0001] The invention relates to a culture medium of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, has broad market prospects due to its comprehensive health care functions and unique pharmacological effects. The cultivation of Cordyceps militaris is generally to put the strains of Cordyceps militaris into liquid culture medium to make liquid strains, and then plant them. But when Cordyceps militaris is planted with the tissue separation method for 2-3 generations, the bacterial strain can degenerate, seriously affecting the yield, and even not growing fruiting bodies. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a culture medium for the strain of Cordyceps militaris, so that the degenerated strain of Cordyceps militaris can grow fruiting bodies through cultivation, so as to increase the planting yield. [0004] The culture medium of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 崔学恒
Owner 崔学恒
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