Microbial solid inocula, and preparation and use thereof

Abstract

The invention mainly relates to microorganism fermenting methods, in particular to a method which prepares a solid FGR microbial inoculum that combines yeast fusion strain (F306), geotrichum candidum link (G361) and rhizopus stolonifer strain (R) by utilizing wastewater and waste residue generated in food industries as well as application thereof. A solid culture medium of the solid microorganismmicrobial inoculum comprises following dry components by weight percentage: 50 to 80 percent of scum residue, 15 to 30 percent of corncob powder, 8 to 12 percent of bran coat, 0.5 to 0.8 percent of corn meal, 0.1 to 3 percent of ureophil and 0.2 to 4 percent of ammonium sulfate, fresh wastewater of bean curd, bean vermicelli, corn starch or yam starch is added for leading the weight percentage ofwater to be 45 to 65 percent, limewater with the concentration of 1 percent is used to adjust pH value between 5.5 and 6.5, a mixed strain liquid of the yeast fusion strain, the geotrichum candidum link and the rhizopus stolonifer strain with the weight 8 to 12 percent of the total dry components is added and completely agitated, ventilated agitation and fermentation are implemented in a fermenting pool with dual purposes of fermenting and drying at the temperature of 28 to 32 DEG C for 18 to 24 hours, and hot air is led into the fermenting pool at the temperature of 40 to 60 DEG C for drying, thus producing the solid microbial inoculum that has great application effect in addition agents of beast and bird feedstuff.

Description

Technical field: [0001] The present invention mainly relates to the fermentation method of microorganisms, in particular to the method and application of using the waste liquid and residue produced in the food industry to prepare the FGR solid inoculum of yeast fusion bacteria (F306), Geotrichum candidum (G361) and Rhizopus (R) three bacteria combination . Background technique: [0002] The preparation method of traditional microbial bacterial agent, used raw material is glucose, peptone, molasses, beef extract, vitamin, inorganic salt and water and is mixed with liquid culture medium, scrapes bacteria lawn from test tube slant strain and inoculates in liquid culture medium, Prepare liquid bacteria by aerobic or anaerobic culture or inoculate liquid bacteria on solid medium composed of corn flour, bran, soybean flour, seed cake, soybean meal and rice bran, etc., and ferment to prepare solid bacteria agent. Its disadvantages are: the cost of raw materials used is high, the ...

AI Technical Summary

Problems solved by technology

Its disadvantages are: the cost of raw materials used is high, the production process is complicated, and energy cannot be saved.

[0003] Most of the microbial strains used in the preparation of traditional microbial strains are: strains isolated and screened from national or local strain banks or directly from nature. The disadvantages are: 1. The activity of microbial strains is poor; 2. To organic matter The ability to decompose and transform is weak; 3. The bacterial agents prepared by these strains have a small number of viable cells, most of which are within 10 8 pcs / g

Its disadvantages are: complex preparation process, long fermentation cycle, high cost and limited application

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