Process for the separation of hydrogenogen of pentose and hexose at high temperature

Abstract

The invention discloses an isolating method of high-temperature hydrogen bacteria of pentaglucose and hexose, relating to an isolating method of high-temperature hydrogen bacteria. The isolating method solves the problems in the existing isolating method of hydrogen bacteria that separation is only carried out under the condition of comparatively low temperate and the isolated hydrogen bacteria is only applied to the fermentation and the hydrogen production of hexose (mainly glucose). The isolating method includes the step: 1. bacteria suspension A is prepared; 2. the bacteria suspension after enrichment is prepared; 3. the bacteria suspension after enrichment is diluted in multiple proportions and then a tube is rolled, and the suspension is cultured till the occurrence of bacterial colony in the tube; 4. bacteria suspension B is prepared; 5. the bacteria suspension B is diluted in multiple proportions and then a tube is rolled, the suspension B is cultured till the occurrence of bacterial colony in the tube and then single bacterial colony is picked for culture again; and 6. the step 3 to step 5 are repeated for five times and then the isolation can be carried out. The isolating method of high-temperature hydrogen bacteria of pentaglucose and hexose can realize isolation at high temperature and the isolated hydrogen bacteria are not only applied to the fermentation and the hydrogen production of hexose but also to the fermentation and the hydrogen production of pentaglucose.

Description

technical field [0001] The invention relates to a method for isolating high-temperature hydrogen-producing bacteria. Background technique [0002] Lignocellulosic raw materials are the most abundant and cheapest renewable resources on earth. Lignocellulosic biomass produced by photosynthesis in the world is as high as 100 billion tons every year, and 89% of it has not been utilized by humans. my country is also very rich in lignocellulosic raw materials. There are more than 700 million tons of crop straw alone every year. In addition to the huge amount of forestry fiber waste and industrial fiber waste, the total amount of lignocellulosic raw materials available every year can reach more than 2 billion tons. The cellulose in the lignocellulosic raw material accounts for about 35% to 45% of the dry weight, and the hemicellulose accounts for about 20% to 40%. The existing methods can hydrolyze the lignocellulosic raw material into fermentable sugars. In the solution, there a...

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Problems solved by technology

The cellulose in the lignocellulosic raw material accounts for about 35% to 45% of the dry weight, and the hemicellulose accounts for about 20% to 40%. The existing methods can hydrolyze the lignocellulosic raw material into fermentable sugars. In the solution, there are mainly two components of hexose (mainly glucose) and pentose (mainly xylose); however, the existing separation methods can only be separated under relatively low temperature conditions, and the separated hydrogen-producing Bacteria can only use hexose (mainly glucose) to ferment hydrogen to produce hydrogen, resulting in low utilization rate of lignocellulosic raw materials and low hydrogen production rate (hydrogen production is only 1200-1365mL / L)

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