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Production of iminodiacetic acid by microorganism catalytic processes and bacterial strain thereof

A technology of iminodiacetic acid and iminodiacetonitrile is applied in the field of microbial catalytic production of iminodiacetic acid and strains thereof, and achieves the effects of less waste water output, high conversion rate and mild reaction conditions

Active Publication Date: 2011-05-25
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem that this method exists is: use chemical hydrolysis to prepare 1 ton of iminodiacetic acid and acidify with concentrated hydrochloric acid to produce at least 6 tons of waste water
[0024] At present, there is no report on biocatalytic hydrolysis of iminodiacetonitrile to produce glyphosate intermediate iminodiacetic acid at home and abroad

Method used

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  • Production of iminodiacetic acid by microorganism catalytic processes and bacterial strain thereof
  • Production of iminodiacetic acid by microorganism catalytic processes and bacterial strain thereof
  • Production of iminodiacetic acid by microorganism catalytic processes and bacterial strain thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Shake flask fermentation culture of Alcaligenes faecalis CCTCC No: M 208168.

[0062] Prepare shake flask fermentation medium A, B, C, D respectively, and its medium composition is as follows:

[0063] A: Ammonium acetate 0.5%, yeast extract 0.3%, K 2 HPO 4 0.3%, MgSO 4 0.01%, FeSO 4 0.001%, NaCl 0.05%, and adjust the pH to 7.0 with 2M hydrochloric acid or 2M sodium hydroxide.

[0064] B: Ammonium acetate 1%, yeast extract 0.5%, K 2 HPO 4 0.5%, MgSO 4 0.02%, FeSO 4 0.003%, NaCl 0.1%, and adjust the pH to 7.5 with 2M hydrochloric acid or 2M sodium hydroxide.

[0065] C: Ammonium acetate 2.0%, yeast extract 0.5%, K 2 HPO 4 0.8%, MgSO 4 0.05%, FeSO 4 0.003%, NaCl 0.03%, adjust the pH to 8.0 with 2M hydrochloric acid or 2M sodium hydroxide.

[0066] D: Ammonium acetate 2.0%, yeast extract 0.8%, K 2 HPO 4 0.8%, MgSO 4 0.1%, FeSO 4 0.01%, NaCl 0.5%, and adjust the pH to 8.5 with 2M hydrochloric acid or 2M sodium hydroxide.

[0067]Pour the...

Embodiment 2

[0070] Embodiment 2: Fermentation tank fermentation culture of Alcaligenes faecalis (Alcaligenes faecalis) CCTCC No: M 208168

[0071] Preparation of seed medium: ammonium acetate 1%, yeast extract 0.5%, K 2 HPO 4 0.5%, MgSO 4 0.02%, FeSO 4 0.003%, NaCl 0.1%, and adjust the pH to 7.5 with 2M hydrochloric acid or 2M sodium hydroxide.

[0072] Prepare fermenter fermentation medium a, b, c, d respectively, and its fermentation medium composition is as follows:

[0073] a: Ammonium acetate 0.5%, yeast extract 0.3%, K 2 HPO 4 0.3%, MgSO 4 0.01%, FeSO 4 0.001%, NaCl 0.05%, and adjust the pH to 7.0 with 2M hydrochloric acid or 2M sodium hydroxide.

[0074] b: Ammonium acetate 1%, yeast extract 0.5%, K 2 HPO 4 0.5%, MgSO 4 0.02%, FeSO 4 0.003%, NaCl 0.1%, and adjust the pH to 7.5 with 2M hydrochloric acid or 2M sodium hydroxide.

[0075] c: Ammonium acetate 2.0%, yeast extract 0.5%, K 2 HPO 4 0.8%, MgSO 4 0.05%, FeSO 4 0.003%, NaCl 0.03%, adjust the pH to 8.0 ...

Embodiment 3

[0081] Embodiment 3: fermented liquid centrifugation or membrane filtration

[0082] Get 2L of the fermented liquid obtained by the fermentation of medium b in Example 2, centrifuge with a low-temperature high-speed centrifuge (at 4°C, centrifuge at 12000rpm for 15min), filter with a hollow fiber membrane (flow rate 150mL / min, pressure 0.8Kg / cm 2 ), roll membrane filtration (flow 150mL / min, pressure 16Kg / cm 2 ), ceramic membrane filtration (flow 150mL / min, pressure 4Kg / cm 2 ) or flat ultrafiltration membrane (Ultra-fla) filtration (flow rate 150mL / min, pressure 3Kg / cm 2 ) method to process the fermentation broth. Then wash the cells with 4L deionized water, collect the wet cells and measure the enzyme activity (measurement method is the same as in the embodiment)

[0083] 1), the results are shown in Table 3.

[0084] Table 3: Wet cell enzyme activity after fermentation broth treatment

[0085] Processing method of fermentation broth

[0086] low temper...

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Abstract

The invention discloses a method for producing diglycolamidic acid, namely, the key intermediate synthesized by glyphosate through biological catalysis and a new strain used in the process. The method takes iminodiacetonitrile as a raw material and a microbial enzyme with nitrilase activity obtained from the fermentation culture of Alcaligenes faecalis ZJUTB10 as a catalyst and obtains the diglycolamidic acid as a product by going through hydrolysis reaction under certain condition. The beneficial effects of the method are mainly reflected by little waste water produced during the reaction process, little pollution, mild reaction conditions, low energy consumption, high conversion rate and high yield and being easy for industrialized production.

Description

(1) Technical field [0001] The invention relates to a method for preparing iminodiacetic acid by catalyzing the hydrolysis of iminodiacetonitrile by microorganisms, and a new bacterial strain used in the preparation process—Alcaligenes faecalis ZJUTB10. (2) Background technology [0002] Glyphosate, English name Glyphosate, trade name Roundup, Nongle, etc., molecular formula C 3 h 8 NO 5 P, molecular weight 169.08, white solid in appearance, insoluble in organic solvents, is a post-emergence systemic non-selective high-efficiency broad-spectrum herbicide, by dissolving the waxy layer on the leaf diameter surface of weeds, the drug effect quickly enters the plant conduction system It has the characteristics of broad-spectrum, low toxicity, no residue, systemic conduction, and excellent killing property. It has no selectivity for plants. Generally, all green plants, whether they are crops or weeds, can be used as medicine. was killed thereafter. In recent years, glyphosate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12N1/20C12R1/05
Inventor 郑裕国柳志强徐建妙薛亚平沈寅初
Owner ZHEJIANG UNIV OF TECH
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