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Method for identifying parent sex of shellfish

A parent and shellfish technology, which is applied in the field of parental gender identification of shellfish without reproductive damage, can solve problems such as difficulties in parental sex identification of dioecious shellfish

Inactive Publication Date: 2009-03-04
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem of difficult gender identification of dioecious shellfish parents in the past, and provides a method for gender identification of shellfish parents. The parent shellfish identified by this method can normally lay eggs and ejaculate sperm. Identified dioecious shellfish parents have general versatility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The first step, the selection of the drill bit diameter of the electric drill: From 2001 to 2003, electric drills were used to determine the gender of the parents of the Philippine clam population in Putian and Dalian. The selected diameter is a drill bit of 0.6mm, and the egg diameter of the Philippine clam is 64-72 μm, so that mature sperm or eggs can be taken out.

[0016] The second step, determination of gonad parts of shellfish: the gonads of Philippine clams are in the visceral mass, and several mature brood shellfish are first dissected to determine the gonad parts, and then drill holes carefully with an electric drill.

[0017] The third step, the treatment of the micro-syringe: Strictly disinfect the micro-syringe with penicillin, especially the needle part, and avoid bringing foreign pathogenic bacteria to the shellfish, so that the shellfish cannot normally lay eggs and ejaculate sperm.

[0018] The fourth step, gender identification under the microscope: pl...

Embodiment 2

[0021] The first step, the selection of the diameter of the drill bit of the electric drill: In 2006, the electric drill was used to determine the gender of the parents of the wide-shelled whole sea bamboo shoots in Zhuanghe, Dalian. Select the drill bit that diameter is 0.6mm for use, and the ovum diameter of the whole sea bamboo shoot of wide shell is 40-42 μ m, can take out mature sperm or ovum like this.

[0022] Step 2: Determination of gonad parts of shellfish: in the visceral mass of the gonads of the wide-shelled whole sea bamboo shoots, first dissect several mature parent shellfish to determine the gonad parts, and then carefully drill holes with an electric drill.

[0023] The third step, the treatment of the micro-syringe: Strictly disinfect the micro-syringe with penicillin, especially the needle part, and avoid bringing foreign pathogenic bacteria to the shellfish, so that the shellfish cannot normally lay eggs and ejaculate sperm.

[0024] The fourth step, gender...

Embodiment 3

[0027] The first step, the selection of the diameter of the drill bit of the electric drill: In 2007, the electric drill was used to identify the sex of the clam parents in Zhuanghe, Dalian. Use a drill bit with a diameter of 0.6mm, and the egg diameter of the green clam is about 90 μm, so that mature sperm or eggs can be taken out.

[0028] The second step, the determination of the gonad part of shellfish: the gonad of the green clam is located in the viscera mass, first dissect several mature brood shellfish to determine the gonad part, and then carefully drill holes with an electric drill.

[0029] The third step, the treatment of the micro-syringe: Strictly disinfect the micro-syringe with penicillin, especially the needle part, and avoid bringing foreign pathogenic bacteria to the shellfish, so that the shellfish cannot normally lay eggs and ejaculate sperm.

[0030] The fourth step, gender identification under the microscope: place the sample taken in the insect culture ...

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Abstract

Disclosed is a method for shellfish parent sex identification, which adopts an electric drill with the diameter of 0.6 mm to ensure a gonad position of a shellfish parent according to the species of a shellfish. The electric drill goes deep into the gonad position to drill on a shell of the shellfish parent, a microsyringe after being disinfected is used to take out sperms or egges, the drops method is adopted under a microscope, a little sea water is added to carry out sex identification, and the egges diverge in the sea water to be of a circular shape or a pear shape under the microscope; the sperms are of a smoke type in the sea water, and are of a tadpole shape under the microscope. The invention has the characteristics of simpleness, being easy to apply and high identification rate. The invnetion has general universality aiming at a gonochorism shellfish parent which cannot be identified in appearance, and the shellfish after being identified can carry out normal propagation, thereby laying a good foundation for developing closely related and distant hybridization as well as family establishment for the shellfish, particularly laying a good foundation for developing population hybridization and large scale production, therefore, the invention has an important guiding significance for genetic improvement of the shellfish.

Description

technical field [0001] The invention relates to a method for gender identification of shellfish parents, in particular to a method for gender identification of shellfish parents without reproductive damage. Background technique [0002] Shellfish are mostly dioecious, and hermaphrodite species are quite common. Although shellfish are divided into dioecious and hermaphroditic, this distinction is not constant. Dioecious species can undergo sex reversal and switch sexes, and hermaphroditic species can also become dioecious due to sex change. Among dioecious species, except for a few species that are dioecious, that is, the secondary sexual characteristics of the two sexes are relatively distinct, and males and females can be distinguished according to external sexual characteristics, most of the dioecious shellfish are dioecious, that is, the secondary sexual characteristics of the two sexes are different. Obviously, it is difficult to identify male and female based on extern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48A61B10/02
Inventor 闫喜武杨凤张国范张跃环霍忠明赵越
Owner DALIAN FISHERIES UNIVERSITY
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