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Method for improving purity of raffinose

A raffinose and purity technology, applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., can solve the problems of easy fouling of separation membranes, long process routes, and many steps, and achieve strong selective adsorption. The effect of industrialized production and simplified purification process

Active Publication Date: 2009-03-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the use of lead acetate will bring heavy metal residues, while the investment of ultrafiltration equipment is relatively large, and there are problems that the separation membrane is easy to pollute and has a short life.
[0010] The usual process of removing monosaccharides and sucrose in the primary product of raffinose is completed simultaneously in the process of crystallization or precipitation, which leads to the need for multiple recrystallizations to obtain high-purity raffinose, which will inevitably reduce the yield of raffinose. Total yield increases production cost and is unfavorable for industrialized production
[0011] In a word, under the existing technology, to obtain high-purity raffinose from defatted cottonseed meal, it is still necessary to obtain the extract solution, decolorization, deproteinization, desalination, removal of monosaccharide and sucrose and crystallization (or recrystallization) respectively through solvent extraction. A series of processes can be realized. In this way, the entire process route is long and there are many steps, and high-yield high-purity raffinose cannot be obtained, which is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Hereinafter, the content of the present invention will be described in detail through examples, but the present invention is not limited thereto. Embodiment 1: prepare raffinose decolorization liquid

[0042] (a) Degreased cottonseed meal (wherein the mass percentage of raffinose is 3.9%) is sieved through a 1.8mm sieve, weighs 435 grams and puts it into a percolation column (Φ3.5cm×70cm), and fills it evenly Its bulk volume is about 560 milliliters, then add 350 milliliters of ethanol aqueous solution with a volume percentage concentration of 75% to the percolation column, soak for 30 minutes at 55°C to fully swell the defatted cottonseed meal, open the outlet valve of the percolation column, and simultaneously The extraction solvent was continuously added to the top of the column, and the flow rate was controlled to be 168 ml / hour until the volume of the percolation extract collected was 1900 ml. The percolating liquid is analyzed, and solid content (solid content re...

Embodiment 2

[0048] Embodiment 2: prepare raffinose crude product

[0049] Put the pretreated HZ816 resin into an adsorption column with a size of Φ5.6cm×97cm. The column bed volume BV is about 2400mL, and the resin is about 1700g inside. The sample loading solution is the raffinose decolorization solution prepared according to the process of Example 1, wherein the solid content is 10%, and the mass percentage of raffinose calculated on a dry basis is 40%. Continuously feed 4BV of raffinose decolorization solution into the fixed-bed adsorption column at a flow rate of 1.5BV / h, and the effluent collected during the sample loading process also contains a small amount of raffinose, which can be directly recycled. After loading the sample, first wash the adsorption column with 2BV deionized water at a flow rate of 2.5BV / h to remove the decolorization solution remaining in the adsorbent voids in the adsorption column, and the obtained washing solution can be recycled; then use 2BV volume percen...

Embodiment 3

[0052] Embodiment 3: prepare raffinose crude product

[0053] Put the pretreated HZ802 resin into an adsorption column with a size of Φ5.6cm×97cm. The column bed volume BV is about 2400mL, and the resin is about 1700g inside. The sample loading solution is the raffinose decolorization solution prepared according to the process of Example 1, wherein the solid content is 10%, and the mass percentage of raffinose calculated on a dry basis is 48%. Continuously feed 3BV of raffinose decolorization solution into the fixed-bed adsorption column at a flow rate of 1.0BV / h, and the effluent collected during the sample loading process also contains a small amount of raffinose, which can be directly recycled. After loading the sample, first wash the adsorption column with 1.5BV deionized water at a flow rate of 1.0BV / h to remove the decolorization solution remaining in the adsorbent voids in the adsorption column, and the obtained washing solution can be recycled; then use 4BV volume perc...

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PUM

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Abstract

The invention discloses a method for improving the purity of raffinose. A fixed bed adsorption separation method is adopted to implement the separation of the raffinose from impurities such as salt, protein, monose, sucrose and the like by utilizing the one-step adsorption separation, so that the mass percentage content of the raffinose in the dry basis is increased from 30%-50% to above 90%, and simultaneously, the loss rate of the raffinose is not more than 3%. The method has the advantages of simple process, convenient adsorbent regeneration, low production cost, short separation period, and easy implementation of the industrial production.

Description

technical field [0001] The invention relates to a method for improving the purity of raffinose, belonging to the technical field of adsorption and separation. Background technique [0002] Raffinose, also known as Melitriose and raffinose, is composed of galactose, glucose and fructose, and is an oligosaccharide and a non-reducing sugar. Research has found that raffinose is an excellent nutrient source for beneficial bacteria such as Bifidobacterium and Lactobacillus acidophilus in the human intestinal tract, which can promote their proliferation, thereby inhibiting the growth of spoilage bacteria in the intestinal tract and reducing the formation of toxic fermentation products . Bifidobacteria ferment raffinose to produce a large amount of short-chain fatty acids, which can stimulate intestinal peristalsis, increase stool moisture and maintain a certain osmotic pressure, thereby preventing constipation. In addition, raffinose can improve the digestive function of the huma...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/06
Inventor 任其龙鲍宗必朱魏吴彩娟苏云邢华斌苏宝根
Owner ZHEJIANG UNIV
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