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Lepista sordida extract as well as preparation method and use thereof

The technology of a mushroom and its extract is applied to the extract of the mushroom and its preparation and application fields, so as to achieve a good inhibitory effect and the effect of inducing resistance.

Inactive Publication Date: 2009-03-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its fruiting body is generally medium in size, rich in protein, and has a high yield in summer and autumn. It is mainly wild in Inner Mongolia, Heilongjiang in Northeast China, and Fujian. There is no report about the active substances in the flower face mushroom against plant viruses.

Method used

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  • Lepista sordida extract as well as preparation method and use thereof
  • Lepista sordida extract as well as preparation method and use thereof
  • Lepista sordida extract as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the acquisition of a kind of flower face mushroom extract and its effect experiment

[0021] 1. Acquisition of the crude extract of Pleurotus chinensis

[0022] 1. Dry the fruiting body of the above-mentioned mushroom in an oven at 30° C., pulverize it, and pass it through a 60-mesh sieve to obtain fruiting body powder of the flowering mushroom.

[0023] 2. Mix 10g of the fruiting body powder obtained in step 1) with 0.025mol / L pH8.0 phosphate buffer at a ratio of 1g / 10ml; after leaching for 4 hours at 4°C, centrifuge at 10,000rpm / min for 20 minutes , collect the supernatant, mix the precipitate with 0.025mol / L pH8.0 phosphate buffer at a ratio of 1g / 10ml, centrifuge to collect the supernatant, combine the supernatants obtained twice to obtain 200ml crude extract things. The protein concentration of the crude extract of this mushroom was detected with a BCA protein concentration kit (Pierce Company, USA), and the determination method was carried out acco...

Embodiment 2

[0049] Embodiment 2, a kind of extraction method of flower face mushroom extract and its TMV inhibition experiment

[0050] The extraction flow chart of the extract of the flower face mushroom of the present invention is as figure 1 As shown, the specific method is as follows:

[0051] 1) Dried the fruiting body of the above-mentioned mushroom in an oven at 20° C., crushed, and passed through a 60-mesh sieve to obtain the fruiting body powder of the flowering mushroom.

[0052] 2) Mix 10g of the fruiting body powder of Pleurotus chinensis obtained in step 1) with phosphate buffer (0.02M, pH7.0) at a ratio of 1g / 20ml; after leaching for 2 hours at 4°C, centrifuge at 10,000rpm / min for 20 minutes , collect the supernatant, mix the precipitate with phosphate buffer (0.02M, pH7.0) according to the ratio of 1g / 20ml, centrifuge to collect the supernatant, combine the supernatants obtained twice to obtain 400ml crude extract thing. The protein concentration of the crude extract of ...

Embodiment 3

[0058] Embodiment 3, a kind of extraction method of flower face mushroom extract and its TMV inhibition experiment

[0059] The extraction flow chart of the extract of the flower face mushroom of the present invention is as figure 1 As shown, the specific method is as follows:

[0060] 1) Dry the fruiting body of the above-mentioned mushroom in an oven at 35° C., pulverize, and pass through a 60-mesh sieve to obtain fruiting body powder of the flowering mushroom.

[0061] 2) Mix 10 g of the fruiting body powder of Pleurotus chinensis obtained in step 1) with phosphate buffer (0.03M pH=6.0) according to the ratio of 1g / 10ml; after leaching for 6 hours at 4°C, centrifuge at 10000rpm / min for 20 minutes, Collect the supernatant, mix the precipitate with phosphate buffer (0.03M PH=6.0) according to the ratio of 1g / 10ml, centrifuge to collect the supernatant, combine the supernatants obtained twice to obtain 200ml crude extract of Pleurotus chinensis. The protein concentration of ...

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Abstract

The invention provides an extract of a Lepista sordida, a preparation method and an application thereof. The method comprises the following steps: (1) mixing the Lepista sordida entity powder with a phosphate buffer, the PH value of which is 6-9; leaching at the temperature ranging from 0 to 4 DEG C; centrifuging to extract a supernatant fluid to acquire a crude extract from the Lepista sordida; (2) adding ammonium sulphate in the supernatant fluid to achieve 50 percent of saturation and centrifuging the supernatant fluid after the supernatant fluid stands for 8-16 hours at the temperature ranging from 0 to 4 DEG C; (3) adding ammonium sulphate in the supernatant fluid to achieve 70 percent of saturation, and centrifuging the supernatant fluid after the supernatant fluid stands for 8-16 hours at the temperature ranging from 0 to 4 DEG C; (4) dissolving with distilled water and precipitating, so as to acquire the protein of the Lepista sordida which resists TMV. The extract of the Lepista sordida belongs to the natural extract, is not harmful to the human beings, and is environment-friendly. Furthermore, the purifying method of the extract of the Lepista sordida is very simple, detailed in steps, and easy to operate.

Description

technical field [0001] The present invention relates to a flower face mushroom extract and its preparation method and application, in particular to a flower face mushroom anti-plant virus protein extract, its preparation method and its application in preventing and treating plant virus diseases. Background technique [0002] Plant virus disease is a kind of disease that is difficult to prevent and control in production, known as "plant cancer". It is estimated that the annual losses caused by plant virus diseases in the whole world exceed 15 billion U.S. dollars, among which the damage caused by tobacco mosaic virus (Tobacco mosaicvirus) reaches more than 100 million U.S. dollars (Wu Yunfeng. World Agriculture, 1995, (5) : 35~36). Tobacco mosaic virus (TMV) is a very important plant virus with a wide range of hosts and can harm a variety of plants. At present, there is no very effective control method for tobacco mosaic virus disease. In recent years, it has been found th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/00A01P3/00
Inventor 李兴红严红燕继晔马凤金周莹黄金宝林秀敏
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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