Method for detecting and capturing antibody indirectly marked with nanometer granule and kit thereof

Abstract

The invention provides an antibody detection double-antigen capture method using nanoparticles. A nanoparticle label and a label between the labeled antigens implement an indirect labeling by combining the label on an antigen and a ligand which is labeled on the nanoparticle label and can specifically recognize the label, wherein the labeled antigen is a gene engineering recombined antigen. With the indirect labeling manner, the sensitivity and the specificity of the method can be remarkably improved.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to an antibody detection and capture method for indirectly labeling nanoparticles, and an immunoassay kit prepared by the method. Background technique [0002] For detection of infectious diseases such as Human Cytomegalovirus (HCMV, CMV), Hepatitis E virus (HEV), Hepatitis A virus (HAV) and other pathogens or microorganisms, It is necessary to use immunological methods to detect its IgM. Several methods that are widely used now are: radioimmunoassay, immunofluorescence, ELISA, etc., but they all have shortcomings and deficiencies in varying degrees. [0003] The radioimmunoassay has radioactive contamination, and the detection time is long, requiring professional equipment and professionals to operate, and single-person detection cannot be realized; the detection time of the immunofluorescence method is long, requires professional equipment and professional operation, and is easily inte...

AI Technical Summary

Problems solved by technology

[0010] 3. Since the labeled antigen generally contains a certain amount of miscellaneous proteins, the miscellaneous proteins are also labeled on the marker together with the target protein during the labeling process, resulting in a decrease in the specificity of the labeling complex;

[0011] 4. Conditions such as buffer solution, pH, and ionic strength for direct labeling cannot guarantee the optimal activity conditions of the labeled antigen. Factors such as vigorous stirring, centrifugation, and blocking during labeling are also likely to affect the active epitope of the labeled antigen, resulting in Reduced sensitivity of final detection

[0016] In summary, the markers used in the antibody detection and capture method detection kits based on recombinant antigen nanoparticles are currently using the direct labeling method, which has methodological defects. Therefore, further improvements are urgently needed on this basis. Increase sensitivity and improve specificity of kit assays

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