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Sliding mode heat transfer media plate PCR instrument

A heat transfer medium and plate technology, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as large volume, false initiation, non-specific binding of primers and templates, etc.

Inactive Publication Date: 2012-06-27
GINGKO BIOTECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the power of the cold / heat source is large, the temperature is not easy to control accurately, and it is easy to cause the temperature to exceed the predetermined value.
In order to prevent exceeding the predetermined value, the circuit and program of temperature control are more complicated
Therefore, in actual use, the speed of heating and cooling is also restricted by the precise control of temperature.
[0006] 3) The TE sheet used in the PCR instrument is easily damaged
The main disadvantages brought by the manipulator are the complex structure, large volume and high price of the instrument.
In addition, when the sample is pulled out until it is inserted into the second plate, it will be exposed to the air, which will reduce the temperature of the sample by 1-2 °C, which will have a bad effect on the PCR reaction, especially after annealing, making the The temperature of the sample is lower than the set value, which may cause non-specific binding of the primer to the template and cause mis-priming

Method used

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  • Sliding mode heat transfer media plate PCR instrument
  • Sliding mode heat transfer media plate PCR instrument
  • Sliding mode heat transfer media plate PCR instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] like Figure 1a and 1b The PCR instrument shown has three heat transfer medium plates 1 arranged in a straight line and fixed in a frame 2. The heat transfer medium plate 1 is a simple rectangular block made of aluminum or other materials with good heat transfer. The space between adjacent plates is filled with solid filler 3, and the upper surfaces of the three heat transfer medium plates 1 and the filler 3 form a plane. The frame 2, the heat transfer medium plate 1 and the filler 3 form a mechanical unit, which is placed on a linear track 4 and driven by a linear motor 8 to realize linear movement. The track 4 is fixed on the casing chassis 5, the sample rack 6 is also fixed, and the sample tube 7 is flat-bottomed. After being placed on the sample rack 6, the position of the sample relative to the casing is fixed. Like this, linear motor 8 will designate working board ( Figure 1a In is the first heat transfer medium plate, Figure 1b The middle plate is the second ...

Embodiment 2

[0040] like image 3 As shown, the feature of this embodiment is that the heat transfer medium plate 1 is fixed on the casing, the sample is placed on the sample holder 6, and the sample holder 6 is movable. Same as Embodiment 1, there are three heat transfer medium plates 1, and the heat source is a resistance wire heating element, which is tightly installed under the plates. The heat transfer medium plate is made of aluminum or other materials with good heat transfer. The heat transfer media plate is a simple rectangular block. The space between every two adjacent media plates is filled with solids. From a geometric point of view only, the upper surfaces of the three heat transfer medium plates 1 and the filler 3 form a plane. Three heat transfer medium plates 1 are arranged in a straight line, fixed on a frame 2, form a mechanical whole together with the filler, and fixed on the chassis chassis 5 . The sample rack 6 is placed on a linear track 4 . In this example, the ...

Embodiment 3

[0044] The above two examples employ specially designed flat-bottomed PCR sample tubes, but this is not a requirement. In this embodiment, the PCR tube is a common conical tube, and a thermocouple (thermal coupling) is realized by a PCR tube socket with good thermal conductivity and a preheated heat transfer medium plate. The selection of the shape and material of the PCR tube socket is similar to the medium plate in the first type of PCR instrument described in the background technology, such as Figure 4a and 4b As shown, one side of the PCR tube socket 12 has a tapered hole for inserting the PCR tube, and the opposite side is a plane in contact with the heat transfer medium block 1 . The PCR tube socket in this example is equivalent to the sample rack in Example 1, and the rest of the structure is the same as Example 1. Since the PCR tube socket has a non-negligible thermal inertia, when it touches the medium plate, it will absorb a non-negligible amount of heat, making t...

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Abstract

The invention discloses a sliding type heat transfer media plate PCR instrument, comprising a machine shell, a plurality of heat transfer media plates which can set the temperature in advance and a sample holder for placing a sample tube, wherein, a plurality of heat transfer media plates are arranged below the sample holder, the vertical distances between the upper surfaces of the heat transfer media plates to the sample holder are equal, the heat transfer media plates and the sample holder can be correspondingly slid under the action of a transmission device, the sliding trajectory is on the same plane, and the sample tube is indirectly or directly contacted with the upper surfaces of the different heat transfer media plates which set the different temperatures in advance according to acertain procedure during the sliding process. The PCR instrument can avoid the plugging-in and pulling-out operations of the sample tube, thereby simplifying a mechanical mobile device, simplifying acontrol circuit and a structure, reducing the size of the instrument, reducing the mechanical faults, reducing the cost and avoiding the harmful excessive temperature decrease of a sample caused by being exposed in air.

Description

technical field [0001] The invention relates to a biological experiment instrument, in particular to an instrument for polymerase chain reaction—PCR instrument. Background technique [0002] PCR reaction is one of the most commonly used molecular biological techniques, and PCR instrument is an essential equipment for PCR reaction. The PCR instrument performs a programmed temperature rise and fall on the sample, and goes through many temperature cycles to achieve the purpose of amplifying the gene. Each temperature cycle of its program usually has 3 temperature points: the first is the denaturation temperature, usually 94-98°C; the second is the annealing temperature, usually 50-65°C, set according to needs; the third Is the extension temperature, usually 68 ~ 74 ℃. At the beginning of the PCR reaction, the thermal cycler heats the sample to the denaturing temperature and holds it there for a few seconds. The sample temperature is then lowered to the annealing temperature ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
Inventor 刘树谟李响
Owner GINGKO BIOTECH BEIJING
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