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Fusion proteins of tick anticoagulant peptide and staphylococcus aureus superantigen-like protein, preparation and application thereof

A technology of anticoagulant peptide and staphylococcus, which is applied in the direction of peptide/protein components, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problem of non-specific activation of the immune system and reduce troubles and pain, improve the level of prevention and treatment, and have good clinical application prospects

Inactive Publication Date: 2009-02-11
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that SSL5 can specifically bind to PSGL-1, compete to antagonize the interaction between PSGL-1 and selectin, significantly inhibit the adhesion of leukocytes, and do not have the non-specific activation of the immune system by standard superantigens

Method used

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  • Fusion proteins of tick anticoagulant peptide and staphylococcus aureus superantigen-like protein, preparation and application thereof
  • Fusion proteins of tick anticoagulant peptide and staphylococcus aureus superantigen-like protein, preparation and application thereof
  • Fusion proteins of tick anticoagulant peptide and staphylococcus aureus superantigen-like protein, preparation and application thereof

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Embodiment Construction

[0029] The amino acid sequence of the TAP-SSL5 fusion protein of the present invention includes a first region having at least 85% identity to the sequence shown in SEQ ID No.1 and a first region having at least 85% identity to the sequence shown in SEQ ID No.2. The second region; wherein: the sequence shown in SEQ ID No.1 is the amino acid sequence of TAP composed of 60 amino acids, and the sequence shown in SEQ ID No.2 is the amino acid sequence of SSL5 composed of 204 amino acids; in the art, Proteins with at least 85% sequence identity generally do not alter the biological activity of the native protein; therefore, the TAP-SSL5 fusion protein of the present invention includes a first region consisting of TAP or a TAP-derived protein and a first region derived from SSL5 or SSL5. These derivative proteins include, but are not limited to: (1) proteins with one or more amino acid residues substituted, deleted or / and inserted, and the substituted or inserted amino acid residues ...

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Abstract

The invention discloses a fusion protein of tick anticoagulant peptide and staphylococcus aureus superantigen-like-protein-5, and an amino acid sequence thereof comprises a first area which has at least 85 percent of similarity to a sequence shown in SEQ ID No.1 and a second area which has at least 85 percent of similarity to a sequence shown in SEQ ID No.2; the invention also discloses a gene for coding the fusion protein, a recombinant expression vector with the gene, a transformant with the recombinant expression vector and a method for preparing the fusion protein; the fusion protein has dual effects of anti-inflammatory and anticoagulation when PSGL-1 is used as a target, and does not affect the normal physiological functions of leucocytes while anti-inflammatory function is performed and does not cause bleeding complications due to excessive or wide anticoagulation while anticoagulation is carried out. The fusion protein can be used for preparing antiatherosclerotic drugs and anticoagulants and is hopeful in greatly improving the prevention and treatment levels of atherosclerosis and thromboembolic complications thereof, and is capable of reducing troubles and pains of sufferers, caused by respective drug administration of antiatherosclerotic drugs and anticoagulants.

Description

technical field [0001] The invention relates to a fusion polypeptide, in particular to a fusion protein of tick anticoagulant peptide and Staphylococcus aureus superantigen-like protein-5, its preparation method and application. Background technique [0002] Cardiovascular and cerebrovascular diseases based on atherosclerosis (ATS) are the main diseases that endanger human health. Its occurrence and development are related to inflammatory response or / and activation of coagulation system, and activation of coagulation system and inflammatory response can promote each other. At present, the therapeutic drugs for ATS only have a single anti-inflammatory or anticoagulant effect. In order to achieve a better therapeutic effect, a combination of drugs is often used clinically, but there are problems such as troublesome administration, patients' easy to miss doses, or poor compliance. So far, there is no research report on anti-ATS drugs with anti-inflammatory and anti-coagulant d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/63A61K38/16A61P9/10A61P7/02
Inventor 胡厚源
Owner ARMY MEDICAL UNIV
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