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Micro array-ELISA detecting kit for detecting six tumor markers

An enzyme-linked immunoassay detection and tumor marker technology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation, increased production cost, and difficulty in popularization, achieves good sensitivity and specificity, and improves work efficiency. , cost saving effect

Inactive Publication Date: 2009-02-04
BEIJING BGI GBI BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of large-scale instrument is expensive to detect and is suitable for large-scale detection. It is not flexible enough to be applied and not easy to popularize.
[0009] In addition, although the immunodiagnostic protein chip can detect multiple indicators at the same time, because it coats the antibody on the substrate used to prepare the protein chip-slides, microspheres or membranes, and the slides and microspheres must be cleaned before use. After pretreatment such as aldylation or polylysine, the production cost increases, and the operation of subsequent experiments is cumbersome, the repeatability is poor, and the market promotion is not strong

Method used

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  • Micro array-ELISA detecting kit for detecting six tumor markers
  • Micro array-ELISA detecting kit for detecting six tumor markers
  • Micro array-ELISA detecting kit for detecting six tumor markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of ELISA plates immobilized with various antibodies in six tumor marker microarray-ELISA kits for detection of various tumor diseases

[0031] In order to detect whether the sample contains characteristic proteins that are indicative of liver cancer, lung cancer, colorectal cancer, gastric cancer, pancreatic cancer, breast cancer, cervical cancer, ovarian cancer, prostate cancer, etc. ELISA plates for antibodies against sex antigens: AFP (alpha-fetoprotein), CEA (carcinoembryonic antigen), CA125 (sugar chain antigen 125), CA19-9 (sugar chain antigen 19-9), CA15-3 (sugar chain antigen 15-3), PSA (prostate specific antigen).

[0032] 1. Antibody Preparation

[0033]Said targeting characteristic antigens: AFP (alpha-fetoprotein), CEA (carcinoembryonic antigen), CA125 (sugar chain antigen 125), CA19-9 (sugar chain antigen 19-9), CA15-3 (sugar chain antigen 15- 3), the antibody of PSA (prostate-specific antigen) is commercially purchased from Fitzgerald Industr...

Embodiment 2

[0039] Example 2 Determination of six tumor marker microarray-enzyme-linked immunoassay kits for tumor disease detection

[0040] 1. Add sample

[0041] Use sample diluent (2.9g disodium hydrogen phosphate, 0.2965g sodium dihydrogen phosphate, 10g sodium chloride, 2.5g Casein, 5g aminopyridine, 100ml calf serum, 75ml glycerol, Tween-205ml, Triton- 1005ml, ProClin300 0.15ml, purified water added to 1000ml) 50ul of the sample to be tested diluted according to 1:21 was added to each reaction well of the microplate plate prepared as in Example 1 above, and placed in an incubation shaker, 3 gear 37 °C for 60 minutes.

[0042] 2. Plate washing:

[0043] After the sample reaction, take the reaction plate out of the oscillator, put it into the corresponding position of the plate washer, adjust the "Number of Strips" button to the corresponding number of bars, and adjust the "Number of Times" button to 5 times. No soaking, no shaking, no pauses. After washing the plate, pat the pla...

Embodiment 3

[0049] Comparison of Six Tumor Marker Microarray-ELISA Kits with the Prior Art Using the methods described in Examples 1-3 above, the samples obtained from clinical units such as Peking University First Hospital were tested. Compared with the commonly used detection methods at present, the results are as follows.

[0050]

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Abstract

The present invention relates to a six-item tumor labeling material micro array (Array)-enzyme linked immunosorbent assay (ELISA) detection kit, in particular to a six-item tumor labeling material micro array (Array)-enzyme linked immunosorbent assay (ELISA) detection kit which is used for detecting the characteristic protein which has an indicating function to the liver cancer, lung cancer, colorectal cancer, stomach cancer, pancreatic cancer, breast cancer, cervical cancer, ovarian cancer, prostate cancer and other tumor diseases.

Description

Technical field: [0001] The invention relates to a six-item tumor marker microarray (Array)-enzyme-linked immunosorbent (ELISA) detection kit, especially for detection of liver cancer, lung cancer, colorectal cancer, gastric cancer, pancreatic cancer, breast cancer, cervical cancer Six tumor marker microarray-enzyme-linked immunoassay kits for characteristic proteins that are indicative of tumor diseases such as ovarian cancer and prostate cancer. technical background: [0002] Malignant tumors are frequently-occurring and common diseases that seriously endanger human health. They can affect various organs or tissues of the human body. Malignant tumors with high morbidity or mortality in my country include liver cancer, lung cancer, colorectal cancer, gastric cancer, and pancreatic cancer. , breast cancer, cervical cancer, ovarian cancer, prostate cancer, etc. [0003] The etiology of most malignant tumors is complex and unclear, the onset is insidious, and early diagnosis i...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/545
Inventor 汪建刘斯奇牟峰何静云娄晓敏
Owner BEIJING BGI GBI BIOTECH
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