Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Color development liquid for peroxidase mensuration and preparation method thereof

A color-developing solution and color-developing technology, applied in biochemical equipment and methods, microbial determination/inspection, biological testing, etc., can solve problems such as reduced practicability and limited detection sensitivity

Inactive Publication Date: 2010-06-23
深圳生科原生物有限公司
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The result is that various solvents greatly inhibit the reaction of TMB and peroxide, so that its detection sensitivity is limited in clinical operations, requiring a very high concentration of peroxidase to complete the determination, which reduces the practicability of the invention

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Color development liquid for peroxidase mensuration and preparation method thereof
  • Color development liquid for peroxidase mensuration and preparation method thereof
  • Color development liquid for peroxidase mensuration and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The method for preparing the chromogenic liquid system for the determination of peroxidase in the embodiment of the present invention comprises the following steps:

[0028] (1) Take carbamide peroxide, heavy metal ion complexing agent, and compounded 4-tert-butyl-4'-methoxydibenzoylmethane and add them into the buffer respectively and mix well as storage solution I for subsequent use;

[0029] Dissolve 3.3'5.5'-Tetramethylbenzidine in acetone, dissolve the resulting solution in ethanol, add the ethanol solution into the buffer and mix well, add sodium thiosulfate into the buffer, mix well as Storage solution II for standby;

[0030] (2) Mix storage solution I and storage solution II, adjust the pH value of the buffer solution, and then constant volume.

[0031] Both the storage solution I and the storage solution II are prepared from a buffer solution with a pH range of 2.5-5.5, which is only used as the above-mentioned basic dissolution buffer system, and has a great...

Embodiment 1

[0034] 1. Prepare storage solution I.

[0035] Take 500ml double distilled water. Add 14.72 grams of sodium citrate monohydrate crystals, shake and mix. Use 0.1mol / L hydrochloric acid to adjust the pH value of the solution to 5.0 to make a 0.05mol / L citric acid-sodium citrate buffer

[0036] Weigh 10 grams of EP-110, add it into the citric acid-sodium citrate buffer, shake and mix well.

[0037] Weigh 0.1 g of Bassoon-1789, add it to the citric acid-sodium citrate buffer, shake and mix well.

[0038] Weigh 0.6 g of carbamide peroxide, add it into citric acid-sodium citrate buffer solution, shake and mix well, and use it as storage solution I for subsequent use.

[0039] 2. Prepare storage solution II.

[0040]Take 300ml of double distilled water, add 4.1g of sodium acetate trihydrate crystals, shake and mix. Use 0.1mol / L hydrochloric acid to adjust the pH value of the solution to 5.0. Prepare 0.05mol / L acetic acid-sodium acetate buffer solution.

[0041] Weigh 0.5 g of ...

Embodiment 2-13

[0046] The preparation process of the color development system with different component concentrations in Examples 2-13 is the same as that of Example 1, only the corresponding amount of the substance is added in the corresponding link of the preparation process. Refer to Table 1 for the final concentration of each component in the chromogenic liquid system solution of the example.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a visualization reagent architecture that is used for peroxydase measurement and a preparation method thereof. The visualization reagent architecture comprises urea peroxide, a heavy metal ion complexing agent and a compound of 4-tert-butyl-4'-methoxy-dibenzoylmethane, 3.3' 5.5'-tetramethylbenzidine and sodium hyposulfite, the preparation method of the architecture comprises the following steps: (1) a storage liquid I is prepared and ready for use, and a storage liquid II is prepared and ready for use; and (2) the storage liquid I and the storage liquid II are mixed, pHvalue of a buffering liquid is adjusted and volume is limited. Peroxides and chromophoric substrates in the visualization reagent architecture that is provided by the invention can coexist stably without reaction for a long time, thus leading the solution architecture to be easily stored and have consistent sensitivity during measurement.

Description

technical field [0001] The invention relates to a chromogenic liquid system for measuring peroxidase and a preparation method thereof. Background technique [0002] The peroxidase chromogenic solution system is a commonly used chromogenic tracer system in the research and detection fields of immunology and immunohistochemistry. At present, it is widely used in clinical and scientific research. The basic principle is: peroxide decomposes in the presence of peroxidase, and releases oxidized oxygen (O-), oxidized oxygen (O-) and the colorless hydrogen donor in the color system ( Chromogen) redox reaction occurs, the chromogen is oxidized, and the chromogen in the oxidized state produces color. After the reaction is completed, the color depth of the chromogenic system directly indicates the content of the detected substance in the reaction system. To achieve qualitative or quantitative detection. However, due to the high standard potential difference between the peroxide and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52C12Q1/28
Inventor 张鹏唐晓玲张阳
Owner 深圳生科原生物有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com