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Production method of lactobacillus micro-ecological preparation for cultivation water regulation

A micro-ecological preparation, aquaculture water quality technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cultivation cost, large equipment investment, and high environmental condition requirements, and achieve lower production costs and lower costs. The effect of energy consumption and turning waste into treasure for reuse

Inactive Publication Date: 2009-01-28
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are interlayers inside the standard fermentation tank, which mainly uses water vapor or cold water to heat or cool down. This kind of culture tank has high requirements on environmental conditions, disinfection and sterilization must be thorough, and the cost of cultivation is high, and the investment in equipment is large.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of production method of the lactic acid bacteria probiotics that is used for cultivating water quality regulation, comprises the following steps:

[0021] (1) Test tube culture: Inoculate two strains of lactic acid bacteria into two test tubes containing MRS medium respectively, and activate the culture at 28°C; then combine the obtained culture solutions;

[0022] (2) Shake flask culture: the activated culture solution of bacterial classification is inoculated in the Erlenmeyer flask that MRS medium is housed with the inoculation amount of 5% of the total weight of culture medium, 28 ℃ of temperature static cultures;

[0023] (3) Seed tank cultivation: the nutrient solution in the triangular flask is inoculated in the polyethylene bottle that the medium of 4500ml is housed with the inoculum amount of 5% of the total weight of the medium, left standstill at temperature 35 ℃ for 24 hours to carry out the first grade Seed culture, fill the bottle; then the primary ...

Embodiment 2

[0028] A kind of production method of the lactic acid bacteria probiotics that is used for cultivating water quality regulation, comprises the following steps:

[0029] (1) Test tube culture: Inoculate two strains of lactic acid bacteria into two test tubes containing MRS medium respectively, and activate the culture at 29°C; then combine the obtained culture solutions;

[0030] (2) Shake flask culture: the activated culture solution of bacterial classification is inoculated in the Erlenmeyer flask that MRS medium is housed with the inoculum amount of 4% of the total weight of culture medium, 29 ℃ of temperature static cultures;

[0031] (3) Seed tank cultivation: the nutrient solution in the triangular flask is inoculated in the polyethylene bottle that the medium of 4500ml is housed with the inoculum amount of 4% of the total weight of the medium, left standstill for 30 hours at a temperature of 30°C to carry out the first grade Seed culture, fill up bottle; Then the primary...

Embodiment 3

[0036] A kind of production method of the lactic acid bacteria probiotics that is used for cultivating water quality regulation, comprises the following steps:

[0037] (1) Test tube culture: inoculate two strains of lactic acid bacteria into two test tubes equipped with MRS medium respectively, activate and cultivate at 30°C; then combine the obtained culture solutions;

[0038] (2) Shake flask culture: inoculate the activated culture solution of bacterial classification in the Erlenmeyer flask that MRS medium is housed with the inoculation amount of 3% of the total weight of culture medium, 30 ℃ of temperature static culture;

[0039] (3) Seed tank cultivation: the nutrient solution in the triangular flask is inoculated in the polyethylene bottle that the medium of 4500ml is housed with the inoculum amount of 3% of the total weight of the medium, left standstill at temperature 25 ℃ for 36 hours to carry out the first grade Seed culture, fill up bottle; Then the primary seed ...

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Abstract

The invention discloses a production method of lactobacillus microecologics which is used for regulating and controlling culture water quality, comprising the following steps of: (1) tube culture; (2) shaking culture; (3) seed tank culture; and (4) fermentor culture: culture solution in the seed tank is inoculated into the fermentor filled with culture solution, and placed for culturing of 24 to 36 hours at the temperature of 25 to 35 DEG C; when the final pH of the fermenting liquid is 3.0 to 4.0 and the density of the lactobacillus is 5 billion / ml, the fermentation is stopped; the pH of the culture solution of the step (3) and the step (4) is 5.5 to 6.5; the culture solution comprises the following ingredients in 1L of water by weight portion: 3 to 5 portions of molasses, 3 to 5 portions of yeast extract, 0.8 to 1 portion of corn steep liquor, 0.1 to 0.3 portion of KH2PO4, 0.3 to 0.5 portion of MgSO4, 3 to 5 portions of NaCl, 8 to 10 portions of glucose and 0.8 to 1 portion of peptone; the method adopts a totally open culture mode to carry out the production of lactobacillus microecologics which is used for regulating and controlling the culture water quality, and is characterized by little equipment investment, little energy consumption, simple technique and low production cost.

Description

technical field [0001] The invention relates to a production method of lactic acid bacteria, in particular to a production method of a lactic acid bacteria microecological preparation used for water quality control of cultivation. Background technique [0002] Lactic acid bacteria are closely related to human beings, and have a wide range of applications. In clinical medicine, lactic acid bacteria, as microecological preparations, can treat intestinal dysfunction and maintain the balance of intestinal bacteria. Applied in feed, it can promote growth and development, prevent and control diseases and improve the quality of poultry products; it can be used as a food additive to increase acidity, improve flavor, increase nutrients and improve nutritional value, prolong food shelf life and prevent corruption. It is widely used in the process of intensive prawn farming. It has obvious effects in regulating algae phase and purifying water quality, which improves the success rate of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/225
Inventor 杨莺莺梁晓华洪敏娜陈永青李佳卓文国樑曹煜成
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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