Preparation of high polymer rich in trace amount chloramphenicol

A high-molecular polymer, chloramphenicol technology, applied in the field of bioengineering, can solve the problems of poor specificity of microbial methods, complicated processing process, slow analysis speed, etc., achieve the effect of less organic solvent, simplify operation steps, and reduce the introduction of impurities

Inactive Publication Date: 2009-01-28
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection limit of ELISA and RIA analysis methods is generally 0.1 ~ 1μg / kg, and the sample pretreatment is simple, the detection throughput is high, but there are false positive results; microbial methods have the disadvantages of poor specificity and low sensitivity; chromatography is internationally recognized Quantitative (trace) detection method for chloramphenicol residues, but it has the disadvantages of complicated sample pretreatment process and slow analysis speed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of preparation method of the macromolecule polymer enriched in trace amount of chloramphenicol, the steps of preparation method are:

[0018] (1). Accurately measure 5mL of tetrahydrofuran, 107uL of 4-vinylpyridine, and 1891uL of ethylene glycol dimethacrylate into a glass ampoule, and ultrasonically treat for 7 minutes to obtain a prepolymerization solution;

[0019] (2). After mixing the pre-polymerization solution, add 30 mg of initiator azobisbutyronitrile (AIBN), sonicate for 10 minutes, fill with nitrogen and deoxidize for 5 minutes, seal it in a round-bottomed container, place it in a constant temperature water bath, and react at 60 ° C After 24 hours a blocky polymer was produced.

[0020] (3). The 250mL mixed solution prepared by the prepared block polymer with methanol: glacial acetic acid in a ratio of 4: 1 was extracted in a Soxhlet extractor for 16h to remove unreacted reagents; vacuum-dried at 80°C, 8h. After taking it out, grind it in a mortar an...

Embodiment 2

[0024] (1). Accurately measure 30mL of tetrahydrofuran, 537uL of 4-vinylpyridine, and 9456uL of ethylene glycol dimethacrylate in a glass ampoule, and ultrasonically treat for 8 minutes to obtain a prepolymerization solution;

[0025] (2). After mixing the pre-polymerization solution, add 150 mg of initiator azobisbutyronitrile (AIBN), ultrasonication for 10 minutes, nitrogen flushing and deoxidation for 10 minutes, seal, place in a constant temperature water bath and react at 60 ° C for 24 hours, and prepare Obtain block polymer;

[0026] Others are with embodiment 1.

[0027] Determination example 2:

[0028] The operation steps are the same as those in Application Example 1, and the enrichment factor of the polymer is finally determined to be 359.

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Abstract

The invention relates to a preparation method of a heavy polymer that has rich trace amount chloromycetin. The pore-foaming agent adopts tetrahydrofuran; the functional monomer adopts 4-vinylpyridine; the cross linker adopts ethylene glycol dimethacrylate, wherein, the mol ratio of the functional monomer and the cross linker is 1:2.5 to 3; the 4-vinylpyridine and ethylene glycol dimethacrylate are dissolved in the tetrahydrofuran, mixed evenly and then the evocating agent zaodiisobutyronitrile is added; the heavy polymer that has rich trace amount chloromycetin is then obtained after ultrasonication, deoxidation by nitrogen blowing, thermostatic water bath reaction, Soxhlet extraction and vacuum drying processes. The heavy polymer made by the invention is economical and practical with simple synthesis process, can be applied to pretreatment of chloromycetin detection in food, provides novel rich materials for detection fields and has great utilization value in practice.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a preparation method of a high-molecular polymer enriched with trace amounts of chloramphenicol in food. Background technique [0002] Chloramphenicol (chlroamphenicol, referred to as CAP) is a broad-spectrum antibiotic produced by Streptomyces venezuelae, and it is also the first antibiotic produced by chemical synthesis. It can inhibit the synthesis of bacterial proteins, especially for leather Lambert-negative bacteria effect is obvious. Because CAP has many harmful effects on the human body, many countries now strictly prohibit the application of CAP in the feeding and treatment of edible animals, that is, the maximum residue limit (MRL) of CAP in edible animal tissues and animal-derived foods is 0. At present, the detection methods of CAP mainly include enzyme-linked immunoassay (ELISA), radioimmunoassay (RIA), microbiological method and chromatography. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08L35/02C08F222/14C08F226/06G01N30/08
Inventor 王硕赵立杰方国臻王俊平张燕
Owner TIANJIN UNIV OF SCI & TECH
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