Air planktonic bacteria detected by scrubbing method and special detecting bottle therefor

Abstract

The invention discloses a novel method for detecting air borne microorganism and a special detection bottle for realizing the method. The detection bottle mainly comprises a transparent bottle body a and a sealing bottle cap b provided with a pumping hole c and a sampling tube e, wherein the sampling tube e is inserted into the bottom of the bottle body; and the upper part of the sampling tube isprovided with a sampling port d, while the bottom is of a porous structure. Before detection, water or a liquid culture medium is arranged in the detection bottle a, and the bottle cap b is covered on the bottle; moreover, the bottom of the sampling tube e is immersed; and the sampling port d and the pumping hole c are corked up and then are sterilized by high-pressure steam for further use. During actual detection, the detection bottle a is positioned in a detected area, and the pumping hole c is connected with a suction device provided with a flowmeter and a timer; the sampling port d is opened, and the suction device is turned on; air in the detected area is cleaned by water or the liquid culture medium e, and then is discharged by means of the sampling tube; when sampling is finished,a coagulating agent is added and stirred evenly; the pumping hole c and the sampling port d are corked up; and finally, cultivation and count are carried out. The method has the advantages of simple operation, accurate result and low cost.

Description

technical field [0001] The invention relates to a new method for detecting airborne bacteria and a special detection bottle for realizing the method. Background technique [0002] At present, the detection method of air planktonic bacteria is mainly realized by impact method. The principle of this method is to use an air pump to blow the air in the detected area to the surface of the culture medium through a slit or a porous plate, and use the tiny weight of the microorganism itself and the inherent tiny stickiness of the culture medium surface to capture some living microbial particles, and after cultivation Colonies were formed and then counted. The biggest disadvantage of this method is that only part of the living microbial particles may be captured, while the other part may not be captured, and may be blown away. Obviously, the accuracy of using the impact method to detect the content of planktonic bacteria in the air is not high. Contents of the invention [0003] ...

AI Technical Summary

Problems solved by technology

The biggest disadvantage of this method is that only part of the living microbial particles may be captured, and the other part may not be captured, and may be blown away. Obviously, the accuracy of using the impact method to detect the content of planktonic bacteria in the air is not high

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