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Ainsliaea fragrans extract and active component thereof as well as their quality control method

A quality control method and technology of Xingxiang rabbit ear wind, applied in the directions of medical preparations containing active ingredients, preparation of organic compounds, plant/algae/fungus/moss components, etc., can solve the chemical composition and Insufficient pharmacological research, unclear research on active ingredients, etc.

Inactive Publication Date: 2009-01-21
上海中创医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in general, so far, the chemical composition and pharmacological research of this apricot rabbit ear wind extract with increased activity is not thorough enough, and the research on its specific active ingredients in the treatment of chronic cervicitis and other gynecological inflammatory diseases I don't know

Method used

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  • Ainsliaea fragrans extract and active component thereof as well as their quality control method
  • Ainsliaea fragrans extract and active component thereof as well as their quality control method
  • Ainsliaea fragrans extract and active component thereof as well as their quality control method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of Apricot Fragrant Rabbit Ear Wind Extract

[0048] Weigh 1 kg of apricot rabbit ear wind medicinal material, heat and decoct 3 times with 20 times the amount of water (8 times, 6 times, 6 times, soak for half an hour before the first decoction), decoct for 1 hour each time, filter, and the filtrate Combine and concentrate to an amount equivalent to 1g of medicinal material per 1ml (concentration temperature 65-70°C). AB-8 macroporous resin (column bed volume about 5L) was applied to the concentrated solution, and the sample was placed for 4 hours to allow the adsorption to balance. First, it was eluted with distilled water until the eluent was basically colorless, and then eluted with 95% medical alcohol (about 20L), collect the ethanol eluent, concentrate, and dry to obtain about 70g (extraction) of Xingxiang rabbit ear wind extract.

Embodiment 2

[0049] Embodiment 2: Separation and purification of compound 1-6

[0050] Part of the apricot rabbit ear wind extract (about 60g) obtained in Example 1 was added to the silica gel to mix the sample at about 1:3, and the column chromatography under reduced pressure (thin-layer chromatography silica gel H, chemically pure, 250g), followed by With 4000ml of chloroform, 1500ml of chloroform-methanol (3:1), 1500ml of methanol, and 3000ml of methanol-formic acid (95:5) for elution, the eluted parts of each ratio were collected and evaporated to dryness to obtain 1.9g of chloroform-eluted part, chloroform-formic acid (95:5) Methanol (3:1) eluted fraction 16.8g (T3-2) and methanol and methanol-formic acid (95:5) eluted fraction 24.6g. Medium-pressure column chromatography on T3-2 (49mm×230mm, TLC 2 analysis of silica gel H about 200g), followed by elution with different ratios of chloroform-methanol (99:1-65:35, pure methanol) to obtain leaching Cream T3-2-1~T3-2-10 has a total of 10...

Embodiment 3

[0056] Embodiment 3: the quality control method of apricot rabbit's ear wind extract

[0057] Preparation of the test solution: Get 50.5 mg of the obtained Apricot Fragrant Rabbit Earwind extract (extract) in Example 1 and put in a 10ml volumetric flask, add methanol to constant volume, and make fully dissolved to obtain the test solution above ultrasonic 10min. Store in the refrigerator until ready to use.

[0058] Preparation of the reference substance solution: Weigh 10.80 mg of compound 5 into a 10 ml volumetric flask, add methanol to constant volume, and shake well to obtain the reference substance solution C5-h1. Precisely measure C5-h12.000ml and add methanol to a 5ml volumetric flask to obtain the reference substance solution C5-h0.4, precisely measure C5-h11.000ml and add methanol to a 10ml volumetric flask to obtain a reference substance solution C5-h0. 1. Weigh 12.00mg of compound 1 and 25.99mg of compound 8 into the same volumetric flask, add methanol to constant...

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Abstract

The present invention relates to a Frangrant Ainsliaea Herb extract and active components, as well as the quality control method. The Frangrant Ainsliaea Herb extract contains sesquiterpene lactones in formula (1) and chlorogenic acids in formula (2). In formula (1), R1 is hydrogen or glucosyl group, R2 is hydrogen or hydroxyl and R3 is methane or methyl; R1, R2 and R3 can be freely combined in the respective selective group categories. In formula (2), R1, R2 and R3 can be coffeoyl or hydrogen. The above two components can be applied in the quality control of Frangrant Ainsliaea Herb medicinal material or the extract.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to an extract of apricot and rabbit ear wind and a quality control method thereof. Background technique [0002] Ainsliaea fragrans Champ (Ainsliaea fragrans Champ) is a commonly used folk herbal medicine. It is the dried whole herb of Ainsliaea fragrans Champ. , edema, carbuncle swollen poison and other diseases. It can be used to treat leucorrhea caused by betting of damp heat, manifested as excessive leucorrhea, yellow and sticky; chronic cervicitis sees the above syndromes. Distributed in Jiangsu, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Guangdong and other places. It is widely distributed in Jiangxi Province, China, from south to north, and sometimes has a certain advantage in the community (growing in patches), and its rich resources are worthy of development and utilization. [0003] Clinically, chronic cervicitis has the characteristics of being di...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P17/04C07H15/26C07H1/08G01N30/36G01N21/47C07D307/93C07C67/56C07C69/732C07C51/47C07C61/08
Inventor 蔡金娜李兴尚刘江云
Owner 上海中创医药科技有限公司
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