Method for preparing Bacillus Subtilis fermented mixed feed for birds
A Bacillus subtilis, mixed feed technology, applied in the direction of animal feed, animal feed, additional food elements, etc., can solve problems such as spread and human harm, and achieve the effects of simple production process, growth promotion, and nutritional composition improvement.
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Embodiment 1
[0022] A preparation method for poultry bacillus subtilis fermented mixed feed, comprising the steps of:
[0023] (1) Use broth culture medium as seed medium, cultivate Bacillus subtilis under 40 DEG C of aerobic conditions for 18 hours, and become first-class seeds;
[0024] (2) inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt the broth medium as the secondary seed medium, and cultivate it for 18 hours under aerobic conditions at 40° C. to become secondary seeds;
[0025] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, using broth medium as the fermentation medium, under 40 ° C aerobic conditions, adopt timing or continuous fed-batch fermentation for 18 hours, and become tertiary seeds;
[0026] (4) After mixing the rice bran, bran and crushed corn with a mass ratio of 1:1:1, they are evenly mixed with the third-grade seed liquid, and the mass ratio of the rice bran, bran and crushed corn mixed with the t...
Embodiment 2
[0029] A preparation method for poultry bacillus subtilis fermented mixed feed, characterized in comprising the following steps:
[0030] (1) Use broth medium as seed medium, cultivate Bacillus subtilis under 30 DEG C of aerobic conditions for 30 hours, and become first-class seeds;
[0031] (2) Inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt the broth medium as the secondary seed medium, and cultivate it under aerobic conditions at 30° C. for 30 hours to become secondary seeds;
[0032] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, using broth medium as the fermentation medium, and under 30°C aerobic conditions, adopt timing or continuous fed-batch fermentation for 36 hours to become tertiary seeds;
[0033] (4) After mixing rice bran, bran and crushed corn with a mass ratio of 1:5:1, they are uniformly mixed with the third-grade seed liquid, and the mass ratio of the rice bran, bran and crushed corn m...
Embodiment 3
[0036] A preparation method for poultry bacillus subtilis fermented mixed feed, characterized in comprising the following steps:
[0037] (1) Use broth medium as seed medium, cultivate Bacillus subtilis under aerobic conditions at 45°C for 8 hours to become first-class seeds;
[0038] (2) Inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt the broth medium as the secondary seed medium, and cultivate it under 45° C. aerobic conditions for 8 hours to become secondary seeds;
[0039] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, using broth medium as the fermentation medium, under 45 ° C aerobic conditions, adopt timing or continuous fed-batch fermentation for 8 hours, and become tertiary seeds;
[0040] (4) After mixing rice bran, bran and crushed corn with a mass ratio of 1:1 to 5:1 to 5, they are evenly mixed with the third-grade seed liquid, and the rice bran, bran and crushed corn are mixed with the third...
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