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Molecule marker of red peel gene of weedy rice

A molecular marker, weedy rice technology, applied in the field of molecular genetics, can solve the problems of strong grain shattering, aggravating the prevalence and flooding of weedy rice, and harm to rice production, and achieves the effect of accurate identification and distinction, and convenient identification.

Inactive Publication Date: 2009-01-07
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0004] Weedy rice is the main hazard factor for rice production in direct-seeding paddy fields. Its morphological characteristics are completely consistent with cultivated rice at the seedling stage, and it is difficult to find early. Weedy rice has strong vitality, strong tillering ability, and competes with cultivated rice for nutrition. Most of them have early earing and shattering. It is strong and harmful to rice production. At the same time, a small amount of weedy rice seeds are mixed into cultivated rice. On the one hand, it affects the appearance and quality of rice. serious hazard

Method used

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  • Molecule marker of red peel gene of weedy rice
  • Molecule marker of red peel gene of weedy rice

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1, the acquisition of a molecular marker for identifying weedy rice

[0025] 1 test material

[0026] Using the weedy rice W16 collected from Yangzhong, Jiangsu, the japonica wide-compatibility variety 02428, and its hybrid F 1 , F 2 group.

[0027] 2 peel color identification

[0028] Randomly take 10 mature seeds of the material, use a rougher to remove the seed shell or directly peel off the shell by hand, and check the color of the seed peel.

[0029] 3 Red Peel Genetic Analysis Populations

[0030] In 2005, the weedy rice with red peel was collected from Yangzhong, numbered W16, and the reciprocal hybrid F 1 , planted F in 2006 1 and harvest F 2 Seed, planted F in 2007 2 Populate 200 plants, and take the leaves at the tillering stage to extract DNA, and harvest F 3 seed. When W16 is used as the female parent, the hybrid F 1 The color of the seed peel is red; when 02428 is used as the female parent, the hybrid F 1 Seed peel color is white, F ...

Embodiment 2

[0052] Example 2: Marker genotype analysis of weedy rice and cultivar resources in the Yangtze and Huaihe River basins using RID14 marker

[0053] The weedy rice materials include 3 copies of Lianyungang Ludao, 1 copy each of Huaiyuantang Rice, Lai'antang Rice, Quanjiaotang Rice and Feidongtang Rice in Anhui Province, which are preserved by the Variety Resource Room of the Institute of Grain and Crop Research, Jiangsu Academy of Agricultural Sciences, and this In recent years, the research team has collected 17 materials of weedy rice in Yangzhou, Nantong, Zhenjiang, Taizhou, and Yancheng, Jiangsu.

[0054] Other materials used for analysis include Asian cultivated rice (Oryza Sativa L.) (indica, japonica) and common wild rice (Oryza Rufipogen), the ancestor of cultivated rice; geographical distribution includes China, India, Korea, the United States, Thailand There are a total of 340 species resources. These materials include not only cultivated varieties popularized in a la...

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Abstract

The invention relates to a molecule marking method for a red peel gene of weed rice and belongs to the field of molecular genetics. A rice type of 02428(white peel) is utilized to be hybridized with Yangzhong weed rice (red peel) to confirm that the red peel gene of the Yangzhong weed rice is a dominance single gene which is decided by a maternal gene; a reciprocal cross result shows to have no cytoplasm effects. An InDel marker RID14 is designed according to the gene structure of the red peel gene Rc of the cloned common wild rice; the InDel marker is co-separated with the red peel gene of the weed rice. The marker can be utilized to accurately and effectively identify the red weed rice and the derived line thereof from the cultivated rice and can provide a molecule basis for the identifying of the weed rice.

Description

technical field [0001] The invention provides a molecular marker identification method for the red pericarp of weedy rice, belongs to the field of molecular genetics, and is specially used for the molecular identification of the red pericarp genotype of weedy rice. technical background [0002] Rice Oryza sativa L. is one of the most important food crops in the world, providing food for nearly half of the world's population. However, rice production is now increasingly endangered by weeds, especially weedy rice (Oryza sativa L.f spontaneous), which belongs to the same biological species as it. Weedy rice is a malignant weed widely distributed in rice fields all over the world, which can reduce the yield and quality of rice. Weedy rice is also known as red rice because of its often red peel. Because weedy rice is closely related to cultivated rice, weedy rice is more difficult to control than other weeds in paddy fields. [0003] Due to the lack of water resources and rura...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 杨杰仲维功陈志德王军
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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