Bivalent vaccine for pig and preparation method thereof

Abstract

The invention provides a divalent vaccine for pigs, wherein, the active ingredients are porcine aftosa inactivation antigen and porcine reproductive and respiratory obstrucion syndrome inactivation antigen with the proportion of 1 to 0.6 - 1.5. The invention also provides a preparation method of the divalent vaccine for pigs, which comprises the processes that monolayer cell is cultured by a rolling bottle, the basic virus and the virus culture fluid are accessed, the Ph value of the culture fluid is regulated to be 7.4 plus or minus 0.2 by adopting an HEPES or NaHCO3 buffer system and the accession amount of the virus and the culture fluid are 300 to 800 ml / 15L per bottle; the virus fluid is collected after the cytopathic effect appears and the virus is inactivated after the potency of the virus is measured; 50V of adjuvant and the mixed inactivation antigen are emulsified according to the volume ratio of 1 to 1, and an 11 model white oil or Marcol 52 model oil and the mixed inactivation antigen are emulsified according to the volume ratio of 1 - 2.0 to 1 for the purpose of confecting the divalent vaccine for pigs. The divalent vaccine of the invention can be put into super-large scale production without restricting the use of virus strain, and can ensure the immune efficacy while the immune dosage is reduced.

Description

Technical field: [0001] The invention relates to a dual vaccine for pigs and a preparation method thereof, in particular to a dual inactivated vaccine of porcine foot-and-mouth disease (FMD) and porcine respiratory and reproductive syndrome (Porcine Reproductive and Respiratory Syndrome) and a preparation method thereof. Background technique: [0002] Foot-and-mouth disease, as a Class A animal epidemic prescribed by the International Veterinary Office (OIE), is internationally recognized as the most harmful animal infectious disease to animal husbandry. Therefore, the research of foot-and-mouth disease vaccine has been highly concerned by researchers at home and abroad. Although Porcine Respiratory and Reproductive Disorder Syndrome (PRRS) is a Class B animal epidemic prescribed by the International Veterinary Bureau, the virus has mutated in my country in the past year, producing highly pathogenic strains, causing a large number of pig deaths and severely injuring my count...

AI Technical Summary

Problems solved by technology

Because porcine foot-and-mouth disease vaccine and porcine respiratory and reproductive disorder syndrome vaccine currently use different adjuvants, the former uses biphasic adjuvant, the latter uses monophasic adjuvant and preliminary tests show that biphasic adjuvant is not suitable for the latter