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Application of enterobacter aerogenes in microbe power generation and its power generation method

A technology of Enterobacter aerogenes and microbial power generation, applied in the field of environment and new energy, can solve the problems of narrow fuel utilization spectrum, undiscovered electrogenic activity of Enterobacter aerogenes, loss of electrogenic activity, etc., and achieves strong applicability

Inactive Publication Date: 2008-12-10
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as MFC anode catalysts, they have certain defects: 1) they belong to strictly anaerobic bacteria, and the presence of oxygen will make them inactivate or lose their electrogenic activity, which is not conducive to large-scale cultivation and practical application of bacterial strains; 2) the fuel utilization of most bacterial strains The spectrum is narrow, and only small molecular weight organic acids such as acetic acid and lactic acid can be used as electron donors (that is, fuels) to generate electricity
After searching the literature and patents, no report on the electrogenic activity of Enterobacter aerogenes has been found

Method used

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  • Application of enterobacter aerogenes in microbe power generation and its power generation method
  • Application of enterobacter aerogenes in microbe power generation and its power generation method
  • Application of enterobacter aerogenes in microbe power generation and its power generation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Validation of the electrogenic activity of Enterobacter aerogenes

[0070] (1) Construction of microbial fuel cells

[0071] In this example, a microbial fuel cell using Enterobacter aerogenes to generate electricity is constructed according to the prior art and methods, as shown in the attached figure 1 As shown, it includes four parts: anode chamber, cathode chamber, exchange membrane and external circuit. Common carbon felt is used as the anode electrode, platinum-coated carbon paper is used as the cathode electrode, and the cathode and anode are connected by wires.

[0072] (2) Inoculate the slant of Enterobacter aerogenes XM02 into conventional LB liquid medium, activate the bacteria on a shaker at 30°C and 200 rpm for 16 hours, and stop culturing after reaching the exponential growth phase. Centrifuge at 8000 rpm at 4°C for 10 minutes at high speed, wash twice with 0.85% sterile saline, resuspend in 0.85% sterile saline, and use it as the microbial fue...

Embodiment 2

[0080] Example 2 Electricity production experiment using glucose as fuel by Enterobacter aerogenes

[0081] Experimental method and steps are the same as in Example 1.

[0082] Preparation of anolyte (b):

[0083] (1) preparing liquid A, the method is the same as in Example 1;

[0084] (2) Prepare liquid B: add 0.18 g of glucose to 100 ml of deionized water;

[0085] (3) Liquid A and liquid B were sterilized according to laboratory routines in this field, cooled to room temperature and mixed to obtain anolyte (b).

[0086] Add 80 milliliters of anolyte (b) in the anode chamber, and inoculate Enterobacter aerogenes, so that the number of bacteria in each liter of anolyte reaches 5×10 5 one, cultured at 30°C; the anode and cathode were connected through a 500-ohm external resistance, and the output voltage was monitored. The results are shown in the attached image 3 shown.

Embodiment 3

[0087] Example 3 Experiment of Enterobacter aerogenes using maltose to generate electricity

[0088] The experimental method and steps are the same as in Example 1, except that the preparation of liquid B is to add 0.36 grams of maltose to 100 milliliters of deionized water; the output voltage changes as Figure 4 shown.

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Abstract

The invention discloses application of enterobacter aerogenes in the aspect of microbe generation and power generating method. The enterobacter aerogenes can be applied to the aspect of microbe power generation as anode catalyst of microbe fuel battery. The power generating method provided by this invention at the same time includes following steps: constructing microbe fuel battery; preparing enterobacter aerogenes inoculum; preparing anode liquid containing fuel; adding the enterobacter aerogenes inoculum and prepared anode liquid into the anode chamber of the microbe fuel battery; performing power generation detection after static cultivation. The enterobacter aerogenes used in the invention belongs to the facultative anaerobe that is easy to be cultured and will not be deactivated under the existing condition of oxygen. The power generation activity can be maintained with low running cost for a long time by periodically adding culture medium. Meanwhile, the invention has wide fuel utilization range, complete biological oxidation and high electronic coefficient of recovery.

Description

technical field [0001] The invention belongs to the technical field of environment and new energy, and specifically relates to the application of Enterobacter aerogenes in microbial power generation, and a power generation method using Enterobacter aerogenes as an anode catalyst to generate electric energy. Background technique [0002] Energy depletion and organic waste disposal are two major problems facing the world today. On the one hand, the depletion of fossil fuels and the increasingly serious problems of global warming have forced countries all over the world to attach great importance to energy issues, and the research and development of renewable new energy is imperative. Building a stable and diverse energy system has become an important part of national strategic security and sustainable development. On the other hand, how to deal with the increasingly large amount of organic waste has become a serious social problem, and the importance of organic waste resource...

Claims

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Application Information

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IPC IPC(8): H01M8/16C12N1/20C12R1/01
CPCY02E60/50
Inventor 周顺桂邓丽芳庄莉
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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