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Method for improving production volume of pyruvic acid preparation of zymotechnics with additive gluconic acid sodium salt

A technology of sodium gluconate and pyruvic acid, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, to achieve the effects of increasing yield, increasing production intensity, and shortening fermentation cycle

Inactive Publication Date: 2008-12-10
JIANGNAN UNIV
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Problems solved by technology

However, by adding a strong oxidizing substrate as a carbon source, increasing the intracellular cofactor NAD + level, the method for improving pyruvate output and production intensity has not been reported in the literature

Method used

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Embodiment Construction

[0008] The bacterial classification used in the present invention is Torulopsis glabrata (Torulopsis glabrata) CCTCC M202019, connects a ring bacterium into seed culture medium (every 500mL Erlenmeyer flask is filled with 50ml culture medium) from fresh slant, shakes flask culture under 30 ℃, 200rpm After 24 hours, a 10% inoculum amount (v / v) was inserted into the fermentation medium (50 mL medium per 500 mL Erlenmeyer flask) and cultured at 30° C. and 200 rpm for 48 hours.

[0009] Fermentation medium (g / L): glucose 100, ammonium chloride 7, KH 2 PO 4 5. MgSO 4 ·7H 2 O0.8, CaCO 3 40 (add when shaking the flask), trace element solution 10mL, vitamin solution 10mL, pH 5.0, tap water to volume. Among them, trace element solution: CaCl 2 2H 2 O2g, FeSO 4 ·7H 2 O2g, ZnCl 2 0.5g, MnCl 2 4H 2 O12g, CuSO 4 ·5H 2 O0.05g, after dissolving 2mol / L HCl, dilute to 1L. Vitamin liquid: niacin 80mg, thiamine 0.15mg, pyridoxine 40mg, biotin 4mg, riboflavin 10mg, dilute tap water ...

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Abstract

The invention discloses a method for improving the output of pyruvic acid by a fermentation method through adding gluconic acid sodium salt, which belongs to the fermentation preparation of pyruvic acid technical field. The method adopts a strategy that during the aerobic fermentation process of Torulopsis glabrata CCTCC M202019 with glucose as a substrate, 10 to 15g / L of the gluconic acid sodium salt is added as a mixed carbon source when the fermentation is performed for 12 to 16h, which ensures that the output of the pyruvic acid is improved by 11.16 percent and the production period is also reduced to 44h from 48h. The research thought has universal application significance to the production of various important fermented products in industry such as organic acid, amino acid and so on.

Description

technical field [0001] The invention discloses a method for adding sodium gluconate to increase the yield of pyruvate produced by fermentation, and relates to the technical field of pyruvate prepared by fermentation of Torugluta glabrata. Background technique [0002] Pyruvate, also known as 2-Oxopropanic acid, α-Ketopropionic acid or Acetylformic acid, is a colorless to pale yellow liquid with acetic acid aroma and pleasant Sour, is one of the most important α-oxocarboxylic acids. [0003] Since the fermentative production of pyruvate began in the 1950s, the research on pyruvate biosynthesis at home and abroad has become more and more in-depth, involving various aspects such as strain selection, nutritional conditions, vitamins, dissolved oxygen levels, and temperature control. In order to achieve a high degree of uniformity in pyruvate yield, conversion rate, and production intensity, the researchers introduced exogenous metabolic pathways through molecular means, and stu...

Claims

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Application Information

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IPC IPC(8): C12P7/40C12R1/645
Inventor 刘立明董志姚陈坚
Owner JIANGNAN UNIV
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