Uses of smoked plum extract in resisting virus, bacteria, mycoplasma or chlamydia of livestock and poultry
A technology of extracting and avian influenza virus is applied in the field of biomedicine to achieve the effect of solving the problem of food antibiotic residues, low toxicity, and controlling the prevalence of infectious diseases
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Embodiment 1
[0024] Example 1 Preparation of ebony extract or effective fraction
[0025] Crush 1kg of ebony plums, then pass through a 10-mesh sieve, add 4-5kg of water to soak the sieve, decoct 3 times, 2-6 hours each time, collect the filtrate 3 times, concentrate to 1-1.5kg, and then use acetic acid Soak in 3-6 times the amount of ethyl ester, soak for 5-10 times continuously, collect the filtrate, recover ethyl acetate, and obtain the ebony extract;
[0026] After dissolving the ebony extract in methanol, mix it with silica gel 1-1.5 times (weight / weight), let it dry naturally, put it on a silica gel column, and use 100% chloroform, 90% chloroform (chloroform / methanol=9:1), 80% chloroform (chloroform / methanol=8:2), chloroform / methanol (2:1), chloroform / methanol (1:1), chloroform / methanol (1:1) / 1% formic acid, chloroform / methanol (1:1) 2) / 1% formic acid; collect 100% chloroform eluted fraction to obtain active ingredient I; collect 90% chloroform eluted fraction to obtain active ingre...
Embodiment 2
[0031] Example 2 Preparation of ebony extract granules and oral liquid
[0032] 100g of ebony extract was dissolved with 500ml of 95% ethanol, and 20g of sodium polyacrylate, 20g of sodium carboxymethylcellulose and 1200g of dextrin were added. Add medicinal starch 660g again, make granule 2000g according to conventional method.
Embodiment 3
[0033] Example 3 Preparation of oral liquid of ebony extract
[0034] Dissolve 100g of ebony extract in 500ml of 95% ethanol, filter with a 100-mesh filter cloth, add 100g of sodium carboxymethylcellulose, mix well, dilute to 2000ML with water and pack into 100ML / bottle of oral liquid.
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