Preparation method of microbial fermentative feedstuff
A microbial fermentation and feed technology, applied in animal feed, animal feed, applications, etc., can solve problems affecting the economic and social benefits of aquaculture, damage to the micro-ecological balance of normal flora, and endogenous infections in animals, to achieve Eliminate the use of antibiotics, improve social and ecological benefits, and reduce the effect of gastrointestinal diseases
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[0037] 1. Preparation of 10kg molasses hydrolyzate:
[0038] Mix 8kg of water, 2kg of molasses, 0.004kg of phosphoric acid, and 0.0025kg of sulfuric acid, then fully stir and dilute, boil with steam for 30 minutes, adjust the pH value to 4.8, let it stand for 10 minutes, and filter it. liquid.
[0039] 2. Composition of activation and expansion medium (g / kg):
[0040] a. Candida utilis
[0041] Molasses acidification solution 200g, ammonium nitrate 5g, potassium dihydrogen phosphate 2.5g, disodium hydrogen phosphate 2.5g.
[0042] b. Geotrichum candidum
[0043] Wort medium.
[0044] c. Streptococcus faecalis
[0045] molasses acidified liquid 100g, yeast extract 3g, potato extract (200g potato extract).
[0046] 3. Expansion process and parameters
[0047] a, Candida utilis fermented liquid (4kg)
[0048] Add 400ml of activated bacteria solution to 4kg of fermentation medium, culture at 28-30°C, 6r / min with stirring, and cultivate for 24h.
[0049] B, Geotrichum cand...
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