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Siphoning type blood sugar test paper

A test strip and siphon-type technology, applied in the field of biosensing, can solve the problems of uncontrolled blood sample volume, uncontrollable amount of reaction objects, uncontrollable blood volume, etc., to achieve simple processing and operable process Strong performance and improved quality indicators

Inactive Publication Date: 2008-11-12
胡军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the patient collects the blood sample on the finger, the amount of the extruded blood sample is uncontrollable, and the amount of blood dripped on the reaction area of ​​the test strip is also uncontrollable.
In biochemical reactions, the amount of reaction objects cannot be controlled, which will inevitably cause errors in test results

Method used

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  • Siphoning type blood sugar test paper
  • Siphoning type blood sugar test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1: each component and weight percent thereof of taken working coating are as follows:

[0010] Material

[0011] The above-mentioned test paper is made by screen printing method, and the screen printing not only prints the conductive circuit, but also prints the working coating on the surface of the working electrode. Place the prepared test strip in the supporting tester and apply a working voltage of 320mV to the two sections of the working electrode and the reference electrode to measure different concentrations of glucose. The results show that the blood glucose test strip has good test repeatability after 10 seconds of reaction. and relevance. Compared with the biochemical analyzer, 50 parts of glucose quality control solution and 50 parts of patient blood samples were taken for blood sugar control test, and the correlation coefficient γ reached 0.9672. The relative standard error of the test strip was CV≤6.5%.

Embodiment 2

[0012] Embodiment 2: each component of the working coating and its weight percentages are as follows:

[0013] Material

[0014] The production method is as in Example 1. Place the prepared test strip in a matching tester and apply a working voltage of 300mV to the two sections of the working electrode and the reference electrode to measure different concentrations of glucose respectively. The results show that after 5 seconds of reaction, the display shows Makes electrodes good test repeatability and correlation. Compared with the hospital's biochemical analyzer, 50 glucose quality control solutions and 50 diabetic blood samples were taken for glucose control test, the correlation γ reached 0.975, and the relative standard error of the test strip was CV≤6.9%.

Embodiment 3

[0015] Embodiment 3: each component and weight percent thereof of taken working coating are as follows:

[0016]

[0017] The screen printing production method of biological enzymes is as in Example 1. The test strips made are placed in a supporting tester and a working voltage of 350mV is applied to the two sections of the working electrode and the reference electrode to measure cholesterol at different concentrations. The results show that the reaction takes place within 20 minutes. Seconds later, good test repeatability and correlation were shown for fabricated electrodes. Compared with the hospital's biochemical analyzer, 20 glucose quality control solutions and 20 diabetic blood glucose control tests were taken respectively, and the correlation γ reached 0.967, and the relative standard error of the test strips was CV≤5.7%.

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Abstract

The invention discloses a siphon test paper, which pertains to the technical field of bio-sensor. The substrate of the test paper is printed with a working electrode, a reference electrode, an electrical circuit and an interface port connected with a read-out instrument. The working electrode and the reference electrode are composed of carbon coatings which are printed on the substrate at once and printed with a reaction working coating, the components by weight percentage include: 12 to 16 percent of enzyme, 15 to 20 percent of a conducting medium, 10 to 20 percent of an adhesive and 42 to 60 percent of buffer. Two sides of the reaction coating are bonded with a double-sided adhesive layer which is adhered with a hydrophilic membrane that is convenient for sucking sample blood and can limit the quantity of sample blood. The invention applies a screen printing method to produce the siphon glucose test paper at high speed and on large scale. The quality indexes of the test paper such as repeatability and precision further can limit the quantity of samples owning to the improvement of the sampling and introduction structure of the test paper, thereby improving quality and greatly reducing production cost. Raw materials applied can be obtained from various resources, processing is simple, technique maneuverability is strong and the test paper is suitable for large scale industrial production. Once the test paper of the invention sucks human blood, results can be read out conveniently and visually from a corresponding matching testing instrument.

Description

technical field [0001] The invention relates to a siphon-type blood sugar test paper, in particular to a siphon-type blood sugar test paper matched with a human blood tester for measuring blood glucose, and belongs to the technical field of biosensing. Background technique [0002] With the rapid development of modern bioanalytical testing technology, a class of small mobile handheld testing instruments or testing methods suitable for patients' real-time self-control and rapid and accurate test results, called POCT (Point of Care Testing) abroad, is rapidly developing. develop. Since this type of tester or test means involves multiple technical fields, the production of test papers matching these testers or test means includes relatively high technical content. Take the test strips for blood glucose meters as an example. The blood glucose test strips currently available on the market all use relatively complicated manufacturing techniques. When performing the test, there ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N33/52C12Q1/26G01N27/327
Inventor 胡军
Owner 胡军
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