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High-purity miglitol production process

A miglitol and high-purity technology, which is applied in the production field of high-purity miglitol, can solve the problems of high bacterial culture cost and biotransformation cost, inability to adapt to large-scale industrial production, and large bacterial loss.

Active Publication Date: 2013-06-05
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0029] However, the cost of bacterial culture and biotransformation in this method is high, and the bacterial cells are collected by centrifugation, and the loss of bacterial cells is large, which cannot be adapted to industrialized large-scale production.

Method used

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  • High-purity miglitol production process
  • High-purity miglitol production process
  • High-purity miglitol production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] In a 100L fermenter, according to the following formula D-sorbitol 6.0%; yeast extract 2.4%; KH 2 PO 44.8% is made into culture medium, inserts Miglitol engineering strain seed with the culture medium of 10% inoculum by volume ratio, and be 1:1 (vol:vol) in ventilation rate, stirring speed is 300rpm, 28 ℃ of temperature Under the condition, carry out 24-28 hour fermentation culture.

Embodiment 2-5

[0070] After a large number of bacterial strains have been cultivated by the above method, the above fermentation liquid is put into a microfiltration circulation tank, and the ceramic microfiltration membrane is used to control the temperature conditions of 0°C, 10°C, 25°C, and 55°C, and the inflow pressure of 0 to 4.0bar and 0 ~3.5 bar outflow pressure. Adding purified water to top wash three times during this period can effectively remove impurities while maintaining the activity of the strain, and obtain highly active and relatively pure mycelia 1, 2, 3, and 4.

Embodiment 6-9

[0072] After a large amount of bacterial strains 1, 2, 3, and 4 are obtained by utilizing the above-mentioned method in the present invention, they are dropped into an aqueous solution dissolved with N-(2-hydroxyethyl)-glucosamine in a 100L conversion tank to carry out biotransformation at pH6.0 , the ventilation rate is 1:1.5 (vol:vol), the stirring speed is 400rpm, and the temperature is 28°C, the conversion formula is determined as follows: N-(2-hydroxyethyl)-glucosamine 6.0kg, MgSO 4 ·7H 2 O2.05kg, oxidize N-(2-hydroxyethyl)-glucosamine to: 6-(2-hydroxyethyl)-amino-6-deoxy-α- L-sorbofuranose to obtain transformation solutions 1, 2, 3, and 4.

[0073] The above-mentioned transformation solutions 1-4 were respectively passed through France Delta’s ceramic membrane microfiltration to remove bacteria. During microfiltration, the temperature conditions were 0°C, 10°C, 25°C, 55°C, the inflow pressure was 0-4.0 bar and the pressure was 0-3.5 bar. bar outflow pressure, to obtain...

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Abstract

The invention relates to a method for producing high-purity miglitol. The method comprises the following stages that: Stage a. a miglitol producing strain is obtained through the cultivation of a culture medium consisting of D-sorbierite, a yeast extract and KH2PO4 and microfiltration separation; Stage b. the miglitol producing strain performs biotransformation, microfiltration, hyperfiltration, nanofiltration, and activated carbon decolorization to a substrate to obtain an intermediate of the miglitol; Stage c. hydrogenation reaction, ion fractionating, activated carbon decolorization, desorption, condensation and crystallization are performed to the intermediate of the miglitol obtained in the Stage b to obtain the high-purity miglitol. The adoption of the method can improve the production efficiency of the miglitol to the utmost extent, thereby realizing the industrialization of the high-purity miglitol.

Description

technical field [0001] The present invention relates to the production method of miglitol, more specifically, the present invention relates to a kind of production method of high-purity miglitol, this method can carry out the whole production process under the stable condition, and economical and practical, safe A production method of high-purity miglitol. The present invention can provide a method for efficiently purifying and producing miglitol, and adopts new technologies such as ceramic membrane microfiltration, ultrafiltration, ion exchange, and crystallization to maximize the production efficiency of miglitol, thereby realizing high-purity rice Industrialization of Glitol. Background technique [0002] As we all know, miglitol is an α-glucosidase inhibitor, mainly used in the treatment of type II diabetes (ie, non-insulin-dependent NIDDM), and its function is mainly to reduce the postprandial blood sugar level of patients and reduce the occurrence of diabetic complica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/12C12N1/20C07D211/22C12R1/01
Inventor 周苗孙新强胡三明
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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