Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid

Abstract

The invention discloses a method for repairing cement-based material cracks, as well as a culture solution and a repair nutrient solution. The method for repairing cement-based material cracks by through microorganisms comprises the following steps that: a Bacillus pasteurii strain is inoculate onto a culture medium provided with a urea-containing substrate; shake cultivation is carried out at a temperature of between 25 and 37 DEG C, and then a culture bacteria solution is taken out and centrifuged and has supernatant fluid removed; strain cells are collected through the culture solution; the concentration of the strain cells is controlled in a range of between 2x10<9> and 1x10<11> cell / ml; standard sand, urea and Ca(NO3)2.4H2O mixture are added to each milliliter of strain cell solutionobtained through collection, mixed, stirred into slurry and injected into cement stone cracks; the frequency of the repair nutrient solution injection is not less than two times; finally, maintenanceis carried out. In the culture solution, each liter of culture solution contains 4 to 6 g of peptone, 2 to 4 g of beef extract and 20 to 60 g of urea. The method fully utilizes microbial resources innature; CO3<2-> decomposed out through microbial enzyme can chelate Ca<2+> in a substrate so as to be mineralized and deposit calcium carbonate, and is close in the combination with the substrate andgood in stability.

Description

Method for repairing cracks in cement-based materials by using microorganisms, culture solution and repair nutrient solution technical field The invention relates to a method for repairing cracks in cement-based materials, a culture solution and a repairing nutrient solution, in particular to a method for repairing cracks in cement-based materials by using microorganisms to deposit calcium carbonate, as well as a culture solution and a repairing nutrient solution. Background technique Cement-based materials are currently the most widely used building materials, and their biggest disadvantage is that they are prone to micro-cracks and local damage under the influence of the surrounding environment during the material preparation process and service period. Cracks in concrete are inevitable, and their microscopic cracks are determined by their own physical and mechanical properties. However, if it cannot be repaired in time, it will not only affect the normal use of the mate...

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Problems solved by technology

However, if it cannot be repaired in time, it will not only affect the normal use of the material, but may also induce macroscopic cracks and brittle fracture.

At present, the crack repair materials commonly used in engineering mainly include cement-based permeable crystalline materials, polymer mortar, polymer grouting materials, etc. Although these materials have played an important role in the repair of many structural crack defects, they are still inevitable. However, there are some defects, such as the durability of materials, environmental friendliness, etc., which also greatly limit their application range.

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