Method for simultaneously determining dopamine and ascorbic acid by utilizing modified glassy carbon electrode

A glassy carbon electrode, ascorbic acid technology, applied in the field of electrochemical analysis and detection, achieves the effects of mild modification conditions, enhanced stability and high catalytic activity

Inactive Publication Date: 2008-10-15
SHANGHAI UNIV
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Problems solved by technology

So far there is no literature report on the simultaneous determination of ascorbic acid and dopamine using chitosan composite cetylpyridinium bromide modified glassy carbon electrode

Method used

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  • Method for simultaneously determining dopamine and ascorbic acid by utilizing modified glassy carbon electrode
  • Method for simultaneously determining dopamine and ascorbic acid by utilizing modified glassy carbon electrode

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Embodiment 1

[0016] The process and steps of the assay method in the present embodiment are as follows:

[0017] (1) Firstly, the glassy carbon electrode is placed in the 0.05μm Al 2 o 3 Polish to the mirror surface on the polishing flannelette, and then ultrasonic cleaning pretreatment in distilled water, dilute nitric acid solution, absolute ethanol and double distilled water;

[0018] (2) Prepare chitosan composite cetylpyridinium bromide composite modified glassy carbon electrode: drop chitosan composite cetylpyridinium bromide composite modifier on the pretreated glassy carbon electrode; the modifier The preparation method is: firstly prepare decaethylpyridine bromide aqueous solution, its concentration is 10mM / L; then prepare chitosan aqueous solution, its weight percent concentration is 0.5~1.0%; The volume ratio is mixed, and the volume ratio of the two is: chitosan aqueous solution: cetylpyridinium bromide aqueous solution = 1: 2; stir to mix evenly, and obtain the composite mod...

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Abstract

The invention relates to a method utilizing chitosan to compound hexadecylpyridine bromide to modify a glassy carbon electrode and synchronously determining dopamine and ascorbic acid, which belongs to the electrochemical analysis detection technical field. The invention is mainly to use chitosan to compound hexadecylpyridine bromide to drip on the glassy carbon electrode to modify the glassy carbon electrode to ensure that a layer of even thin-film is formed on the surface of the electrode, and further to improve the stability of the electrode. The preparation method of the compound modified agent obtained by the compounding of the chitosan and the hexadecylpyridine bromide is as follows: firstly, cetylpyridinium bromide water solution with percentage concentration by weight of between 5 and 10mM / L is prepared, then chitosan water solution with percentage concentration by weight of between 0.5 and 1.0 percent is prepared, then the cetylpyridinium bromide water solution and the chitosan water solution are mixed according to certain volume ratio, the volume ratio of the cetylpyridinium bromide water solution and the chitosan water solution is between 1:1 and 3:1, and the composite modified agent can be obtained after the mixture is stirred to fully and evenly mix. The method can be directly used for quick electrochemical determination of dopamine and ascorbic acid, and has the characteristics of quickness, sensitiveness, accuracy, high catalytic property and so on.

Description

technical field [0001] The invention relates to a method for simultaneously measuring dopamine and ascorbic acid by using chitosan compound cetylpyridinium bromide to modify a glassy carbon electrode, belonging to the technical field of electrochemical analysis and detection. Background technique [0002] Electrochemical methods for the determination of dopamine (DA) have attracted widespread interest because it is an important neurotransmitter that plays an important role in the functioning of the central nervous, renal, and endocrine systems. Dysregulation of dopaminergic nerves in the brain is an important cause of schizophrenia and Parkinson's disease. Therefore, the research on its determination method is of great significance to explore its biological mechanism and the diagnosis of related diseases. Likewise, ascorbic acid is a vitamin in the human diet and plays an important role in the treatment of colds, mental illness, infertility, cancer, and AIDS. Dopamine and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 丁亚平曹晓妹罗立强李丽边瑞香邹雪莲
Owner SHANGHAI UNIV
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