Process for abstracting bacterial DNA from phlegm, kit and uses thereof

Abstract

The invention discloses a method and a kit for extracting bacterial nucleic acid from sputa and applications thereof. The method is to use liquefaction reagent to liquefy a sputum sample and add solid particles to lyse cells to extract nucleic acid form the sputum. Overcoming the drawbacks of the prior method including complex procedures, a long period of time, complex operation and inadequate samples, the method of the invention for extracting nucleic acid samples is convenient and quick in operation, adequate in nucleic acid samples, low in cost and easy to implement. The method and kit for extracting bacterial nucleic acid can be used to identify strains of mycobacteria and to detect whether a gene locus of mycobacterium tuberculosis is of a wide type or a mutant type. With a chip for identifying strains and a chip for detecting whether the gene locus of mycobacterium tuberculosis is of a wide type or a mutant type, the method can identify the strains of mycobacteria and to detect whether a gene locus of mycobacterium tuberculosis is of a wide type or a mutant type in a quick, accurate and high-pass way.

Description

A method, kit and application for extracting bacterial nucleic acid from sputum technical field The invention relates to a method, kit and application for extracting bacterial nucleic acid from sputum. Background technique In 1882, Kock discovered that the pathogenic bacteria of tuberculosis was Mycobacterium tuberculosis. In 1946, Hinshaw reported that 100 tuberculosis patients were treated with streptomycin (SM) for the first time, creating a new era of tuberculosis diagnosis and treatment. After more than 100 years of hard work, people have a complete set of experience in the incidence, transmission, prevention, diagnosis, treatment and prognosis of tuberculosis. In the United States and other industrialized countries, tuberculosis decreased at a rate of 5.8-10% per year from 1953 to 1985. The American medical community was once optimistic that tuberculosis that could be prevented and treated would become a scene in American history and would no longer be a Public heal...

AI Technical Summary

Problems solved by technology

This test is usually done on the basis of culture of sputum tubercle bacilli, so it takes longer

(4) Mycobacterium species identification, according to the physical and chemical characteristics of different mycobacteria, can accurately identify mycobacteria species, but the operation is complicated, and the drugs used in individual tests have certain risks

At present, the clinical detection of drug resistance of Mycobacterium tuberculosis is mainly based on culture plus drug sensitivity test. The method is reliable but time-consuming, generally takes 4-6 weeks, and it is difficult to meet the clinical needs

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