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Germfree mice artificial milk and its preparation method

A technology for artificial feeding and mice, applied in the field of dairy products for artificial feeding of animals, can solve the problems of unreasonable nutrition matching, low survival rate of mice, poor growth and development, etc., and achieve low cost, good growth and development, and gastric emptying. quick effect

Active Publication Date: 2008-10-15
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing artificial feeding milk for sterile mice has unreasonable nutritional mix, complicated preparation methods, and high cost. If it is used to feed sterile mice, the survival rate of the mice is low. And other issues

Method used

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  • Germfree mice artificial milk and its preparation method
  • Germfree mice artificial milk and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the preparation of sterile mouse artificial feeding milk (102ml)

[0017] a. Carry out high temperature and high pressure disinfection on the container and filter, the pressure is 1.05kg / cm 2 , the temperature is 121°C, and the time is 30 minutes;

[0018] b. Infant milk powder, based on 100g, its component content is: 16.1g protein, 28.7g fat, 51.0g carbohydrate, 1541.0IU vitamin A, 258.0IU vitamin D, 7.0IU vitamin E, 27.0μg vitamin K1, 518.0μg Vitamin B1, 781.0μg vitamin B2, 340.0μg vitamin B6, 1.8μg vitamin B12, 4445.0μg niacin, 39.6μg folic acid, 2780.0μg pantothenic acid, 12.7μg biotin, 50.0mg vitamin C, 72.0mg choline, 407.0mg calcium, 313.0mg of phosphorus, 68.0mg of magnesium, 8.3mg of iron, 4.1mg of zinc, 357.0μg of copper, 250.9mg of sodium, 587.0mg of potassium, 543.0mg of chlorine; take 45.0g of infant milk powder, put it in a plastic bottle sterilized by step a, and seal it with a stopper , carry out 40KGy Co 60 After irradiation, add 75ml ...

Embodiment 2

[0021] Embodiment 2, the preparation of sterile mouse artificial feeding milk (102ml)

[0022] a. Carry out high temperature and high pressure disinfection on the container and filter, the pressure is 1.05kg / cm 2 , the temperature is 121°C, and the time is 30 minutes;

[0023] b. Infant milk powder, based on 100g, its component content is: 16.1g protein, 28.7g fat, 51.0g carbohydrate, 1541.0IU vitamin A, 258.0IU vitamin D, 7.0IU vitamin E, 27.0μg vitamin K1, 518.0μg Vitamin B1, 781.0μg vitamin B2, 340.0μg vitamin B6, 1.8μg vitamin B12, 4445.0μg niacin, 39.6μg folic acid, 2780.0μg pantothenic acid, 12.7μg biotin, 50.0mg vitamin C, 72.0mg choline, 407.0mg calcium, 313.0mg of phosphorus, 68.0mg of magnesium, 8.3mg of iron, 4.1mg of zinc, 357.0μg of copper, 250.9mg of sodium, 587.0mg of potassium, 543.0mg of chlorine; take 55.0g of infant milk powder, put it in a plastic bottle sterilized by step a, and seal it with a stopper , carry out 40KGy Co 60 After irradiation, add 75ml ...

Embodiment 3

[0026] Embodiment 3, the preparation of sterile mouse artificial feeding milk (102ml)

[0027] a. Carry out high temperature and high pressure disinfection on the container and filter, the pressure is 1.05kg / cm 2 , the temperature is 121°C, and the time is 30 minutes;

[0028] b. Infant milk powder, based on 100g, its component content is: 16.1g protein, 28.7g fat, 51.0g carbohydrate, 1541.0IU vitamin A, 258.0IU vitamin D, 7.0IU vitamin E, 27.0μg vitamin K1, 518.0μg Vitamin B1, 781.0μg vitamin B2, 340.0μg vitamin B6, 1.8μg vitamin B12, 4445.0μg niacin, 39.6μg folic acid, 2780.0μg pantothenic acid, 12.7μg biotin, 50.0mg vitamin C, 72.0mg choline, 407.0mg calcium, 313.0mg of phosphorus, 68.0mg of magnesium, 8.3mg of iron, 4.1mg of zinc, 357.0μg of copper, 250.9mg of sodium, 587.0mg of potassium, 543.0mg of chlorine; take 50.0g of infant milk powder, put it in a plastic bottle sterilized by step a, and seal it with a stopper , carry out 40KGy Co 60After irradiation, add 75ml o...

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Abstract

The invention discloses a milk for artificially feeding a germ-free mouse and a preparation method thereof. The milk contains, per 102 mL, 45.0-55.0 g of infant milk powder, 3.0-5.0 mg of vitamin B1, 0.7-0.9 mg of vitamin B2, 3.0-5.0 mg of vitamin B6, 30.0-50.0 Mug of vitamin B12, 11.0-13.0 mg of vitamin C, and water in balance. The preparation method of the milk comprises the following steps: sterilizing infant milk powder by irradiation, dissolving the sterilized milk powder in sterile water to make milk solution, dissolving vitamins B1, B2, B6, B12 and C in water to obtain vitamin solution, sterilizing by filtration, mixing the vitamin solution with the milk solution, and stirring thoroughly to obtain the milk for artificial feeding of germ-free mouse. The milk for artificially feeding the germ-free mouse has reasonable trophic structure and simple production process, increases the growth development level and survival rate of artificially-fed germ-free mouse, and reduces the cost of artificial feeding.

Description

technical field [0001] The invention relates to milk products for animal artificial feeding, in particular to a sterile mouse artificial feeding milk and a preparation method thereof. Background technique [0002] Experimental animals are the basis and conditions for the development of life sciences. They not only play a very important role in biomedical research, but are also widely used in many fields such as agriculture, chemical industry, environmental protection, aerospace, and commodity inspection. Usually, ordinary animals have parasites inside and outside their bodies, bacteria and viruses in their bodies, and antibodies in their serum. When used in scientific research, there will be various interferences, resulting in inaccurate experimental results. Using sterile animals can To overcome the above shortcomings, to ensure the consistency and reliability of the experimental results. Sterile animals refer to experimental animals that do not carry other living organism...

Claims

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Application Information

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IPC IPC(8): A23C9/152A23C9/158A23K1/18A23K10/20A23K20/174A23K50/50
Inventor 魏泓袁静李文霞牛荣唐欢曾本华
Owner ARMY MEDICAL UNIV
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