Special micro-flow control chip and single sampling continuous separation electrophoresis method

Abstract

The present invention discloses a special microfluidic chip and a single-sampling continuous-separating electrophoresis method. The invention is applied in the technique field of the microfluidic chip in life science, medicine and analytical chemistry. The invention is characterized in that the invention comprises a method for exerting the electrophoresis high tension electricity parameter and a microfluidic chip which is used together with the method. The shunting driving voltage which is exerted to the microfluidic chip through an electrophoresis special high voltage power source leads to that the redundant buffer solution and sample solution totally flow into the shunting solution storage tank through a shunting channel. The side wall of the sampling channel on the special microfluidicchip is added with a shunting channel which forms a crossing structure with the former sampling channel and a shunting solution storage tank communicating with the shunting channel. The beneficial effects of the invention is effective prevention to the leakage of the buffer solution, avoidance to the dilution of the sample solution, evident reinforcement to the repeatability of a plurality of peak heights of the sample in the continuous-separation electrophoresis pattern and increased accuracy of the analyzing result of the continuous-electrophoresis separation experiment.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips used in life sciences, medicine, and analytical chemistry, and relates to a dedicated microfluidic chip and a single-time sample-loading continuous separation electrophoresis method, in particular to the ability to improve continuous separation electrophoresis An electrophoresis method for peak height stability and a microfluidic chip used in conjunction with the method. Background technique [0002] Microfluidic Chip (Microfluidic Chip), also known as Micro Total Analysis System (μ-TAS), integrates the processes of sample preparation, biochemical reaction, separation and detection involved in the fields of chemistry and biology into a digital square Centimeter-sized technology on a chip. When the electrophoresis is separated, a voltage is applied to both ends of the microchannel to control the flow direction and velocity of the fluid in the microchannel under the composite action of e...

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Problems solved by technology

[0003] However, when the continuous electrophoresis separation experiment is carried out on the microfluidic chip with the currently commonly used entrainment sampling method, the buffer solution will leak, and the buffer solution will enter the sample solution pool through the sampling channel, resulting in the dilution of the sample solution and resulting in electrophoresis. The peak heights of multiple samples in the spectrum fluctuate widely, and the stability of multiple peak heights is very poor, which is not conducive to accurate calculation of the component content in the sample, and it is not convenient for rapid, accurate, batch and economical continuous separation and electrophoresis experiments

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