Gas concentration and gas pressure adjustable cell culture case

Abstract

The invention provides a gas concentration and pressure adjustable type cell culture box, comprising a box body, a plastic bag communication pipe and a manometer communication pipe, wherein a side wall of the box body is provided with an air pipe connected with an air bag containing needed gases; the plastic bag communication pipe is communicated with a sealed plastic bag; the manometer communication pipe is communicated with a manometer; the lower part of a side wall of the box body is provided with a water pipe by which water can be drained out of the box or injected in the box, and the top part of the box body is provided with a box cover provided with a seal ring. The gas concentration and pressure adjustable type cell culture box has the advantages that: (1) mixed gases do not need to be pre-prepared, and different gases are directly injected into the cell culture box, which builds all gas conditions needed and saves gases and time, and the operation is easy; (2) the gas pressure in the cell culture box can be stably controlled, thereby the cell culture box can be used for the cell culture of which the gas pressure condition is the environmental atmospheric pressure as well as the research of the influence of the high gas pressure and the low gas pressure on cells; (3) the cell culture box matching a common constant temperature culture box can replace CO2 culture box, thereby saving costs.

Description

technical field [0001] The invention relates to a cell culture box, in particular to a cell culture box with adjustable gas concentration and air pressure. Background technique [0002] At present, when establishing cell culture conditions, the usual method for establishing the gas conditions in the culture container (hereinafter referred to as the cell culture box) is to first prepare the mixed gas as required, and then pass the prepared mixed gas into the culture box to replace the gas in the box. The atmosphere not only wastes a lot of gas and the controllability of the gas concentration is poor, but also ignores the problem of the imbalance between the air pressure inside the box and the surrounding atmospheric pressure. For the study of the influence of high or low pressure factors on in vitro cell culture, there is currently a lack of accurate, economical, simple and feasible methods. Contents of the invention [0003] The object of the present invention is to provi...

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Problems solved by technology

[0002] At present, when establishing cell culture conditions, the usual method for establishing the gas conditions in the culture container (hereinafter referred to as the cell culture box) is to first prepare the mixed gas as required, and then pass the prepared mixed gas into the culture box to replace the gas in the box. Atmosphere, not only wastes a lot of gas, the controllability of gas concentration is poor, but also ignores the problem of imbalance between the air pressure inside the box and the surrounding atmospheric pressure

For the study of the influence of high or low pressure factors on in vitro cell culture, there is currently a lack of accurate, economical, simple and feasible methods

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