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Three-dimensional high-flux medicaments sifting chip and manufacture method thereof

A high-throughput, chip-based technology, applied in biochemical equipment and methods, biomass post-processing, biological material pre-treatment, etc., can solve the problems of large consumption of reagents and drugs, high development cost, and long screening cycle. Achieve the effects of increasing stability and smoothness, reducing channel internal resistance, and easy operation

Inactive Publication Date: 2008-08-20
WUHAN UNIV +1
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Problems solved by technology

However, traditional drug screening is mainly performed in glass petri dishes and 96-well plates, with heavy workload, large consumption of reagents and drugs, long drug screening cycle, and high development costs

Method used

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  • Three-dimensional high-flux medicaments sifting chip and manufacture method thereof
  • Three-dimensional high-flux medicaments sifting chip and manufacture method thereof
  • Three-dimensional high-flux medicaments sifting chip and manufacture method thereof

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Embodiment Construction

[0030] The manufacturing steps of the high-throughput drug screening chip in the present invention are as follows:

[0031] (1) Use the Coredraw drawing software to design the channel graphics as shown in Figure 1 and Figure 2. The cell injection and waste liquid output channels shown in Figure 1 are smooth bifurcated structures, and the cell injection port 1 leads to two cells into the tributary channel Channel 2, each cell is injected into the tributary channel, and two cells are respectively injected into the tributary channel, and the waste liquid outlet 4 leads to three waste liquid branch channels 3 . The channel shown in Figure 2 includes a concentration gradient channel 5, a quantitative cell culture chamber 6, a cell injection hole 7, a waste liquid outlet hole 9, and three groups of cell culture parallel chambers 8 composed of cell culture chambers. , two groups of blank control chambers 10 and positive drug control cell culture chambers 11 . As shown in Figure 3, t...

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Abstract

The invention discloses a three-dimensional high-flux drug screening PDMS-glass chip and the preparation method thereof, which makes uses of micro-electro processing and micro-plastic film technologies to produce the three-dimensional PDMS-glass chip with a two-layer micro-channel structure. A cell injection and waste liquid output channel of a first layer of PDMS chip is bonded with another layer of polydimethyl siloxane chip together, a drug concentration gradient channel and a quantitative cell culture chamber are arranged on the other layer of PDMS chip, the quantitative cell culture chamber is connected with an outlet channel of the drug concentration gradient channel, the quantitative cell culture chamber is respectively connected with a cell sample injection hole drilling position and a waste liquid outlet hole drilling position by the channel; the three-dimensional high-throughput drug screening PDMS-glass chip can overcome the shortcomings of heavy workload, great reagent and drug consumption, long screening period and high development cost of the traditional drug screening technology. The three-dimensional high-flux drug screening PDMS-glass chip can realize miniaturization of the chip, high flux, low cost, small reagent consumption, accurate and reliable test data and the simple design requirements of the operational process in the drug screening application on cell level.

Description

technical field [0001] The invention relates to a novel three-dimensional polydimethylsiloxane-glass chip capable of realizing high-throughput drug bioactivity screening at the cell level and a preparation method thereof, belonging to the technical field of microfluidic chips. Background technique [0002] With the development of microfluidic technology, drug screening has become one of the most potential application fields in current microfluidic chip technology. The analysis and detection of single cells and intracellular trace substances have been preliminarily realized in microfluidic chips, but high-throughput drug screening at the cellular level is still a difficulty and hot spot in the development of microfluidic technology. However, traditional drug screening is mainly performed in glass petri dishes and 96-well plates, with heavy workload, large consumption of reagents and drugs, long drug screening cycle, and high development costs. At present, there is an urgent ...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/02
Inventor 赵兴中王晓明张南刚
Owner WUHAN UNIV
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