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Method for extracting polysaccharide from salvia miltiorrhizae

A technology of Salvia miltiorrhiza and polysaccharide, applied in the field of preparation of polysaccharide compounds, can solve problems such as difficulty in obtaining good effects and the like

Inactive Publication Date: 2008-07-23
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Ganoderma lucidum is a fungus, and the polysaccharides of Ganoderma lucidum are mostly stored in the cell wall of the fruiting body. The main components of the fruiting body are cellulose, hemicellulose and lignin, while Danshen has a tougher hard-walled tissue, which has a greater relationship with Ganoderma lucidum. Different, it is difficult to achieve good results by applying the above method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135]1 Kg of Danshen coarse powder was crushed to a fine powder with a particle size of 10 μm, 8 liters of pure ethanol was added, reflux was extracted for 1 hour, and the filter residue was collected by filtration. Add 10 times of water, add 15 grams of cellulase, adjust the pH value to 5.5 with sodium hydroxide, mix with the filter residue, keep warm at 60°C for 60 minutes, ultrasonicate for 60 minutes, filter, the filtrate is polysaccharide extract, after the extract is concentrated Add ethanol to adjust the alcohol concentration to 90%, let it stand overnight, vacuum-dry the precipitate at 60° C., and pulverize it to obtain 71 g of crude Danshen polysaccharide.

Embodiment 2

[0137] 1 Kg of Danshen coarse powder was crushed to a fine powder with a particle size of 100 μm, 10 liters of 90% ethanol was added, reflux was extracted for 2 hours, and the filter residue was collected by filtration. Add 12 times of water, add 5 grams of cellulase, adjust the pH value to 4.0 with sodium hydroxide, mix with the filter residue, incubate at 80°C for 30 minutes, ultrasonicate for 90 minutes, filter, the filtrate is the polysaccharide extract, and the extract is concentrated Add ethanol to adjust the alcohol concentration to 85%, let it stand overnight, vacuum-dry the precipitate at 70° C., and pulverize it to obtain 70 g of crude Danshen polysaccharide.

Embodiment 3

[0139] 1 Kg of Danshen coarse powder was crushed to a fine powder with a particle size of 50 μm, 10 liters of 90% ethanol was added, reflux was extracted for 2 hours, and the filter residue was collected by filtration. Add 12 times of water, add 10 grams of cellulase, adjust the pH value to 6.0 with sodium hydroxide, mix with the filter residue, keep warm at 40°C for 90 minutes, ultrasonicate for 30 minutes, filter, the filtrate is polysaccharide extract, after the extract is concentrated Add ethanol to adjust the alcohol concentration to 85%, let it stand overnight, vacuum-dry the precipitate at 70° C., and pulverize it to obtain 54 g of crude Danshen polysaccharide.

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Abstract

The invention provides a method to extract a polysaccharide from a Danshen root, which is characterized in that the Danshen root is processed by the superfine pulverizing and degreasing and hydrolyzed in the water with pH 4 to 6 for 0.5 to 1.5 hours under 40 to 80 degree centigrade temperature by one or a multiple of a cellulase, a papain, and a pectase, and then processed for 0.5 to 1.5 hours under an ultrasonic wave with a frequency of 59KHz, filtering, and finally the polysaccharide is precipitated from the filtered liquid with an ethanol. The method to extract a polysaccharide from a Danshen root has the advantages of adjusting the enzyme types and dosage based on the fungus polysaccharide extraction process, regulating the process conditions of enzyme process and the ultrasonic wave extraction, realizing to extract Danshen root polysaccharide in a way of low energy consumption and high efficiency.

Description

technical field [0001] The invention relates to the preparation of polysaccharide compounds, in particular to the extraction of polysaccharides from the Chinese medicinal material Salvia miltiorrhiza. Background technique [0002] Danshen (Salviamiltiorrhiza Bge.) is a well-known drug for promoting blood circulation and removing stasis, which is widely used in the treatment of coronary heart disease, angina pectoris, myocardial infarction and cerebrovascular disease. During the pharmacological screening of the chemical components of Salvia miltiorrhiza, it was found that the polysaccharides extracted from the roots of Salvia miltiorrhiza were used in the experimental nephropathy model induced by aminonucleosides and the liver injury model induced by carbon tetrachloride. Reduce the excretion of urinary protein, inhibit the increase of serum cholesterol and lipid peroxide concentration, improve the ratio of serum albumin to globulin (A / G), and reduce the increase of ALT cause...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 吕志平刘强
Owner SOUTHERN MEDICAL UNIVERSITY
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