Novel method for extracting natural vitamin E, phytosterol, fatty acid methyl ester by enzyme catalysis and molecular distillation

Abstract

The invention relates to a method to extract a natural vitamin E, a phytosterin, and a fatty acid methyl ester by the enzyme catalysis and the molecular distillation, which is characterized in that a triglyceride in a distillate which is deodorized from a vegetable oil is hydrolyzed into a fatty acid through a lipase, a methanol is catalyzed by the lipase and the fatty acid is esterified and generated into a fatty acid methyl ester, and the natural vitamin E and the phytosterin are produced when the methanol and the fatty acid methyl ester are processed by filtering, desolventizing, freezing and removing the phytosterin, and removing the fatty acid methyl ester through the secondary molecular distillation. The the extraction method of natural vitamin E, phytosterin, and fatty acid methyl este, compared with the prior art, has the advantages of adopting the process that the oil is catalyzed and hydrolyzed first and then the enzyme catalyzes the fat to be esterified and to be processed by the secondary molecular distillation, no producing wastewater, shortening the esterification time, increasing the natural vitamin E content, deploying the secondary freezing and crystallization to remove the phytosterin with high recovery rate of phytosterin, and beneficially increasing the purity of the vitamin E.

Description

technical field [0001] The invention relates to a new method for extracting natural vitamin E, phytosterol and fatty acid methyl ester through enzyme catalysis and molecular distillation. Background technique [0002] There are eight isomers of natural tocopherol, namely α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol and α-tocotrienol, β-tocotrienol, γ-tocotriene Phenol, delta-tocotrienol. The eight isomers of natural vitamin E all have optical activity. Among them, the optical activity of (RRR)-α-tocopherol is very small. Specific rotation (in 2,2,4-trimethylpentane) is only +0.16°, specific rotation in ethanol [α] 25 546.1 is +0.32°. Specific rotation of β-tocopherol [α] 25 546.1 is +2.9° (c=7.15 in ethanol). Specific rotation of γ-tocopherol [α] 25 546.1 is +2.2° (c=9.32 in ethanol). δ-tocopherol Specific Rotation [α] 25 546.1 is +3.4° (c=15.5 in ethanol). [0003] Natural vitamin E is a light yellow viscous transparent liquid, odorless and tasteless...

AI Technical Summary

Problems solved by technology

Since vegetable oil deodorized distillates contain more triglycerides, lipase Novozym435, Lipozyme TL, and Lipozyme RM have good catalytic effects on the esterification of free fatty acids and The catalytic effect of alcohol transesterification is poor, and triglyceride cannot be completely converted into fatty acid ester, which makes it difficult to purify vitamin E, and the obtained vitamin E content is not high

It can be seen from the implementation examples of this patent that this method uses three times of esterification, the time is relatively long, and the process is cumbersome in actual production.

In this method, vitamin E is destroyed by conventional distillation methods.

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